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SNAP Lepto Test

This page contains information on SNAP Lepto Test for veterinary use.
The information provided typically includes the following:
  • SNAP Lepto Test Indications
  • Warnings and cautions for SNAP Lepto Test
  • Direction and dosage information for SNAP Lepto Test

SNAP Lepto Test

This treatment applies to the following species:
Company: Idexx Labs.

Canine Leptospira Antibody Test Kit

For veterinary use only.

SNAP* Lepto

In vitro diagnostic for the detection of anti-Leptospira antibodies in canine serum.

Precautions and warnings

● All wastes should be properly decontaminated prior to disposal.

● Do not mix components from kits with different serial numbers.

● Do not use a SNAP device that has been activated prior to the addition of sample.

● This test kit cannot differentiate between antibodies to pathogenic and nonpathogenic serovars of Leptospira.

● This test kit cannot differentiate between antibodies produced as a result of a natural infection with the organism and those produced by vaccination.

● Refer to country specific Material Safety Data Sheet for regional hazard identification.


● Store at 2-8°C until expiration date.

● All components must be at room temperature (18-25°C) before running the test.

Kit Components





1 bottle of Leptospira Antigen:HRPO Conjugate (Preserved with ProClin™ 150)

3.8 mL


SNAP Device

5 or 10

Reagents contained in each device:

Wash Solution (preserved with ProClin™ 150)

0.4 mL

Substrate Solution

0.6 mL

Other Components: transfer pipettes, sample tubes and reagent rack


Conjugate / Wash Solution- H317/H412/P261/P280/P302+P352/P333+P313: May cause an allergic skin reaction. Harmful to aquatic life with long lasting effects. Avoid breathing mist/vapors. Wear protective gloves. IF ON SKIN: Wash with plenty of soap and water. If skin irritation or rash occurs: Get medical advice/attention.

Substrate Solution - H317/P261/P280/P302+P350/P333+P313: May cause an allergic skin reaction. Avoid breathing mist/vapors. Wear protective gloves. IF ON SKIN: Wash with plenty of water. If skin irritation or rash occurs:

Get medical advice/attention.

Sample information

● Samples must be at room temperature 18-25°C before beginning the test procedure.

● Serum, either fresh or stored at 2-8°C for up to one week, can be used.

● For longer storage, serum can be frozen (-20°C or colder) and then recentrifuged before use.

● Hemolyzed or lipemic samples will not affect test results.

Test procedure

1. Allow all components to equilibrate at room temperature 18-25°C for 30 minutes before use. Do not heat.

2. Using the pipette provided, dispense 3 drops of sample into a new sample tube.

3. Holding the bottle vertical, add 4 drops of conjugate to the sample tube.

4. Cap the sample tube and mix it thoroughly by inverting it 3-5 times.

5. Place the device on a horizontal surface. Add the entire contents of the sample tube to the sample well, being careful not to splash the contents outside of the sample well.

The sample will flow across the result window, reaching the activation circle in 30-60 seconds. Some sample may remain in the sample well.

6. When color FIRST appears in the activation circle, push the activator firmly until it is flush with the device body.

Note: Some samples may not flow to the activation circle within 60 seconds, and therefore, the circle may not turn color. In this case, press the activator after the sample has flowed across the result window.

7. Read test result at 10 minutes.

Note: The positive control may develop sooner, but results are not complete until 10 minutes.

Interpreting The Test Results

Positive Result

Any color development in the sample spots indicates the presence of anti-Leptospira antibody in the sample.

Note: A positive result indicates presence of antibodies to any of the following serovars: Grippotyphosa, Canicola, Pomona, and Icterohaemorrhagiae. The sample spot cannot differentiate between the four serovars.

Negative Result

Only positive control spot develops color.

Invalid result

Background - If the sample is allowed to flow past the activation circle, background color may result. Some background color is normal. However, if colored background obscures test result, repeat the test.

No color development - If the positive control does not develop color, repeat the test.

Performance summary by MAT Titer

Symbol descriptions

IDEXX Technical Support

USA/Canada: 1 800 248 2483 •

Australia: 1300 44 33 99 •


VLN/PCN: 313/5052.50

*SNAP is a trademark or registered trademark of IDEXX Laboratories, Inc. or its affiliates in the United States and/or other countries.

Patent information:

© 2021 IDEXX Laboratories, Inc. All rights reserved.


IDEXX, One IDEXX Drive, Westbrook, Maine 04092 USA

CPN: 1116073.2

Telephone:   207-556-0300
Fax:   207-556-4346
Pet Diagnostics/Order Desk:   800-248-2483
Poultry/Livestock Diagnostics:   800-548-9997
Poultry/Livestock Order Desk:   800-943-3999
THIS SERVICE AND DATA ARE PROVIDED "AS IS". DVMetrics assumes no liability, and each user assumes full risk, responsibility, and liability, related to its use of the DVMetrics service and data. See the Terms of Use for further details.

Copyright © 2023 Animalytix LLC. Updated: 2023-11-27