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SNAP* Combo FeLV Ag/FIV Antibody Test Kit (Canada)

This page contains information on SNAP* Combo FeLV Ag/FIV Antibody Test Kit for veterinary use.
The information provided typically includes the following:
  • SNAP* Combo FeLV Ag/FIV Antibody Test Kit Indications
  • Warnings and cautions for SNAP* Combo FeLV Ag/FIV Antibody Test Kit
  • Direction and dosage information for SNAP* Combo FeLV Ag/FIV Antibody Test Kit

SNAP* Combo FeLV Ag/FIV Antibody Test Kit

This treatment applies to the following species:
Company: Idexx

Feline Leukemia Virus Antigen-Feline Immunodeficiency Virus Antibody Test Kit

For veterinary use only.

SNAP* Combo

The SNAP Combo FeLV Ag/FIV Antibody Test is a rapid immunoassay for simultaneous detection of feline leukemia virus (FeLV) antigen and antibody to feline immunodeficiency virus (FIV) in feline serum, plasma or whole blood. The presence of FeLV p27 antigen is diagnostic for FeLV infection, and the presence of specific antibodies to FIV indicates that a cat has been exposed to FIV and may have an active FIV infection.

Kit components





1 bottle, anti-FeLV/FIV Ag: HRPO conjugate

(Preserved with gentamicin and Kathon)

4.5 mL or 8.5 mL


SNAP device

5, 15 or 30

Reagents contained in each device:


Wash solution (preserved with Kathon)

0.4 mL


Substrate solution

0.6 mL

Other components: transfer pipettes, sample tubes and reagent rack


Store devices and test reagents at 2-8°C. All components must be at room temperature (18-25°C) before running the test-do not heat.

Precautions And Warnings

● FIV antigen used in the conjugate has been chemically inactivated.

● Do not mix components from kits with different serial numbers.

● Do not use a SNAP device that has been activated prior to the addition of sample.

WARNING: Conjugate - H316/P332+P313/EUH208. Causes mild skin irritation. If skin irritation occurs: Get medical advice/attention. Contains Kathon. May produce an allergic reaction.

Sample Information

● Samples must be at room temperature (18-25°C) before beginning the test procedure.

● Serum, plasma or anticoagulated whole blood (e.g., EDTA, heparin), either fresh or stored at 2-8°C for up to one week, can be used.

● For longer storage, serum or plasma can be frozen (-20°C or colder) and then recentrifuged before use.

● Hemolyzed or lipemic samples will not affect results.

Test procedure

1. Allow all components to equilibrate at room temperature (18-25°C) for 30 minutes before use. Do not heat.

2. Using the pipette provided, dispense 3 drops of sample into a new sample tube.

3. Holding the bottle vertical, add 4 drops of conjugate to the sample tube.

4. Cap the sample tube and mix it thoroughly by inverting it 3-5 times.

5. Place the device on a horizontal surface. Add the entire contents of the sample tube to the sample well, being careful not to splash the contents outside of the sample well.

The sample will flow across the result window, reaching the activation circle in 30-60 seconds. Some sample may remain in the sample well.

6. When color FIRST appears in the activation circle, push the activator firmly until it is flush with the device body.

Note: Some samples may not flow to the activation circle within 60 seconds and, therefore, the circle may not turn color. In this case, press the activator after the sample has flowed across the result window.

7. Test results must be read at 10 minutes from the time of activation.

Note: The positive control may develop sooner, but results are not complete until 10 minutes.

Interpreting Test Results

Positive Result

Any color development in the sample spots indicates the presence of FIV antibody or FeLV antigen in the sample.

Negative Result

Only the positive control spot develops color.

Reaction With Negative Control

The negative control spot serves as a safeguard against diagnosing false positives, and helps indicate that the assay has been run properly.

Invalid Result

If color in the negative control spot is equal to or darker than the FIV antibody or FeLV antigen sample spot, the test is invalid for that spot.

Invalid Results

Background-If the sample is allowed to flow past the activation circle, background color may result. Some background color is normal. However, if colored background obscures the test result, repeat the test.

No color development-If the positive control does not develop color, repeat the test.

Idexx Snapshot Dx* Analyzer

Test results can also be read using the SNAPshot Dx analyzer. A complete description of how to enter patient data and read test results using the SNAPshot Dx analyzer can be found in the SNAPshot Dx Analyzer Operator’s Guide.

Sensitivity and specificity

Comparison Test

Sample Size
SNAP Combo Test/Reference Test

Sample Type

Relative Sensitivity and Specificity
95% Confidence Limit

Kappa Statistic






PetChek FeLV Ag






Serum/Plasma/Whole Blood

Sen., 98.6% (95% CL 91.8%-100%)

Spec., 98.2% (95% CL 94.5%-99.6%)


PetChek FIV






Serum/Plasma/Whole Blood

Sen., 93.5% (95% CL 81.7%-98.3%)

Spec., 100% (95% CL 97.6%-100%)


Sensitivity and Specificity are based on visual interpretation of SNAP results.

CL = confidence limit

Symbol descriptions

IDEXX Technical Support

USA/Canada: 1 800 248 2483 •

Australia: 1300 44 33 99 •


U.S. Vet. License No. 313

Product Code 502A.02

*SNAP, SNAPshot Dx, and PetChek are trademarks or registered trademarks of IDEXX Laboratories, Inc. or its affiliates in the United States and/or other countries.

Patent information:

© 2017 IDEXX Laboratories, Inc. All rights reserved.

IDEXX LABORATORIES, One IDEXX Drive, Westbrook, Maine 04092 USA

Distributor: IDEXX Canada, 3044 Bloor Street, Toronto, ON M8X 2Y8 Canada


CPN: 1243004.9

Technical Service:   800-248-2483
Telephone:   800-667-3411
Order Desk:   800-248-2483
Fax:   905-475-7609
THIS SERVICE AND DATA ARE PROVIDED "AS IS". Animalytix assumes no liability, and each user assumes full risk, responsibility, and liability, related to its use of the Animalytix service and data. See the Terms of Use for further details.

Copyright © 2023 Animalytix LLC. Updated: 2023-05-29