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SNAP Canine Heartworm Antigen Test Kit

This page contains information on SNAP Canine Heartworm Antigen Test Kit for veterinary use.
The information provided typically includes the following:
  • SNAP Canine Heartworm Antigen Test Kit Indications
  • Warnings and cautions for SNAP Canine Heartworm Antigen Test Kit
  • Direction and dosage information for SNAP Canine Heartworm Antigen Test Kit

SNAP Canine Heartworm Antigen Test Kit

This treatment applies to the following species:
Manufacturer: Idexx Labs.

Canine Heartworm Antigen Test Kit

For veterinary use only.

SNAP* HTWM

In-vitro diagnostic for the semiquantitative detection of Dirofilaria immitis (D. immitis) antigen in canine and feline whole blood, serum or plasma. The SNAP test is an enzyme immunoassay: after conjugate and test sample are mixed and added to the sample well, the device is then activated, releasing reagents stored within the device. Color development in the antigen indicator spots is proportional to the antigen concentration in the sample.

Precautions And Warnings

• During manufacturing, the bioactive spots are dyed for quality-control purposes. This does not interfere with the test results or interpretation.

• All wastes should be properly decontaminated prior to disposal.

• Do not mix components from kits with different serial numbers.

WARNING: Conjugate - H316/P332+P313/EUH208. Causes mild skin irritation. If skin irritation occurs: Get medical advice/attention. Contains Kathon. May produce an allergic reaction.

Storage

• Store at 2-25°C until expiration date.

• All components must be at room temperature (18-25°C) before running the test.

Kit Components

Item

Reagents

Quantity

1

1 bottle anti-HTWM: HRPO conjugate (Preserved with gentamicin and Kathon)

7.0 mL

2

SNAP Device

5, 15 or 30

Reagents contained in each device:

 

Wash Solution (preserved with Kathon)

0.4 mL

 

Substrate Solution

0.6 mL

Other Components: transfer pipettes, sample tubes, and reagent rack

Sample Information

• Samples must be at room temperature (18-25°C) before beginning test procedure.

• Serum, plasma or anticoagulated whole blood (e.g., EDTA, heparin), either fresh or stored at 2-8°C for up to one week, can be used.

• For longer storage, serum or plasma can be frozen (-20°C or colder) and then recentrifuged before use.

• Hemolyzed or lipemic samples will not affect test results.

Test Procedure

1. If stored in a refrigerator, allow all components to equilibrate at room temperature (18-25°C) for 30 minutes. Do not heat.

2. Using the pipette that is provided, dispense 3 drops of sample into a new sample tube.

3. Holding the bottle vertical, add 4 drops of conjugate to the sample tube.

4. Cap the sample tube and mix it thoroughly by inverting it 3-5 times.

5. Place the device on a horizontal surface. Add the entire contents of the sample tube to the sample well, being careful not to splash the contents outside of the sample well.

The sample will flow across the result window, reaching the activation circle in 30-60 seconds. Some sample may remain in the sample well.

6. When color FIRST appears in the activation circle, push the activator firmly until it is flush with the device body.

Note: Some samples may not flow to the activation circle within 60 seconds, and, therefore, the circle may not turn color. In this case, press the activator after the sample has flowed across the result window.

7. Read test result at eight minutes.

Interpreting Test Results

Positive Result

Any amount of color development in the sample spots indicates a positive result and color intensity is proportional to the heartworm antigen concentration in the sample.

Low Antigen Levels

High Antigen Levels

Negative Result

Only the positive control spot develops color.

Invalid Results

1. Negative Control (safeguard against false positives)-If color in the negative control spot is equal to or darker than the color in the sample spots, the result is invalid and the sample should be retested.

2. No Color Development-If the positive control does not develop color, repeat the test.

3. Background-If the sample is allowed to flow past the activate circle, background color may result. Some background color is normal. However, if colored background obscures test result, repeat the test.

Idexx Snapshot Dx* Analyzer

Test results can also be read using the SNAPshot Dx Analyzer. A complete description of how to enter patient data and read test results using the SNAPshot Dx Analyzer can be found in the SNAPshot Dx Analyzer Operator’s Guide.

Sensitivity and Specificity

Comparison Test

Sample Size
Kit/Reference

Sample Type

Relative Sensitivity and Specificity
95% Confidence Limit

Kappa Statistic

+/+

-/+

+/-

-/-

Total

PetChek* Heartworm

152

3

0

103

258

Serum/Plasma/Whole Blood

Sen., 98% (95% CL 94%-100%)
Spec., 100% (95% CL 96%-100%)

0.98

The heartworm sensitivity values in this table were generated from a mixed worm-burden population. Sensitivity for samples from dogs with very low wormburdens (≤2 worms, n=24) was 91.7%; sensitivity when testing samples from dogs with >2 worms was 99.2%.

Sensitivity and Specificity are based on visual interpretation of SNAP results.

CL = confidence limit

Symbol Descriptions

IDEXX Customer Support

USA/Canada 1 800 248 2483 • Europe 00800 1234 3399 • Australia 1300 44 33 99

idexx.com

U.S. Vet. License No. 313

Product Code 5018.03

*SNAP, SNAPshot Dx and PetChek are trademarks or a registered trademarks of IDEXX Laboratories, Inc. or its affiliates in the United States and/or other countries.

Patent information: idexx.com/patents.

© 2014 IDEXX Laboratories, Inc. All rights reserved.

IDEXX LABORATORIES, One IDEXX Drive, Westbrook, Maine 04092 USA

06-04367-10

NAC No.: 1116046.9

IDEXX LABORATORIES, INC.
ONE IDEXX DRIVE, WESTBROOK, ME, 04092
Telephone:   207-556-0300
Fax:   207-556-4346
Pet Diagnostics/Order Desk:   800-248-2483
Poultry/Livestock Diagnostics:   800-548-9997
Poultry/Livestock Order Desk:   800-943-3999
Website:   www.idexx.com
Email:   lpdweb@idexx.com
Every effort has been made to ensure the accuracy of the SNAP Canine Heartworm Antigen Test Kit information published above. However, it remains the responsibility of the readers to familiarize themselves with the product information contained on the US product label or package insert.

Copyright © 2016 North American Compendiums. Updated: 2016-10-31

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