RapidVet-H Companion Animal Major Crossmatch Test KitThis page contains information on RapidVet-H Companion Animal Major Crossmatch Test Kit for veterinary use.
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- RapidVet-H Companion Animal Major Crossmatch Test Kit Indications
- Warnings and cautions for RapidVet-H Companion Animal Major Crossmatch Test Kit
- Direction and dosage information for RapidVet-H Companion Animal Major Crossmatch Test Kit
RapidVet-H Companion Animal Major Crossmatch Test KitThis treatment applies to the following species:
(Donor Blood / Recipient Serum or Plasma)
For use on either canine or feline species
Description and Intended Use: Crossmatch identifies serological incompatibility between a donor and recipient. Performing a crossmatch is an essential procedure to be considered before most transfusions and in addition to blood typing. Crossmatch reveals incompatibilities between a donor and recipient that will not be evident from blood typing alone.
RapidVet-H Major Crossmatch is performed using donor red blood cells and recipient serum or plasma. The test will alert the veterinarian to the existence of antigens on donor red blood cells that correspond to antibodies, whether acquired or naturally occurring, present in the recipient serum or plasma. In an incompatible transfusion, these antibodies can cause a major, life-threatening reaction.
CANINES: Currently in-house testing is readily available only for canine red blood cell antigen DEA 1. Most severe transfusion reactions are based on DEA 1 incompatibility. Commercial testing is available for some DEA antigens but is not available for all antigens or for newly discovered blood types including Dal, Kai-1 and Kai-2.
Not all DEA antigens are problematic for transfusion reactions. Furthermore, the existence of naturally occurring antibodies to DEA antigens and newer named blood types, and the significance of these antibodies in causing adverse reactions, are still being assessed.
Even if donor blood is more completely typed, most recipient blood is not. The major crossmatch enables the recognition of DEA 1 incompatibility following the initial transfusion of DEA 1 positive cells to a DEA 1 negative patient. In addition, the major crossmatch may indicate varying degrees of incompatibility to other unspecified donor red blood cell antigens. The number of different DEA antigens on each red blood cell can affect the size and intensity of agglutination observed in an incompatible crossmatch reaction. (Refer to interpretation section for examples.)
FELINES: As cats have naturally occurring antibodies to antigens not on their red cells, a crossmatch should be performed prior to every transfusion. Cats with Type A blood have antibodies to Type B antigens and cats with Type B blood have antibodies to Type A antigens. New feline red blood cell antigens are being discovered, including Mik and others. Cats lacking Mik antigens on their red cells will have Mik antibodies before any transfusion. Thus determining only the A or B blood type for compatibility is not sufficient. Only a crossmatch will uncover the problem if the antibody titers are sufficient.
Kit Contents: Instructions; Procedure Diagram; Photo Identifier/Crossmatch Centrifuge List; Report Cards; 3 Test Stands each containing 7 tubes; and 3 pipette bags each containing 10 pipettes.
Donor Sample: 0.1 ml (100 µl) EDTA anticoagulated whole blood, OR 0.05 ml (50 µl) packed red blood cells (pRBCs).
Recipient Sample: 1.0 ml serum or plasma obtained by centrifuging 2.0 ml whole blood.
Gel tubes should remain upright at all times. A Procedure Diagram and Photo Identifier/Crossmatch Centrifuge List for use with all tests is included in each kit. Do not discard.
A. Remove: 1 test stand containing 7 tubes, 1 pipette bag and 1 report card.
B. Write Donor name/ID on all seven (7) tubes.
C. Write Recipient name/ID on Yellow Top REACTION TUBE and Clear Top REACTION GEL tube (yellow-bordered labels)
D. Insert Blue Top BLOOD PREP TUBE upright into well provided in test stand.
Test Procedure: [Follow bracketed numbers on Procedure Diagram]
Use a clean pipette for every step to prevent contamination.
 PIPETTE Donor Sample: 2 drops (100 µl) whole blood OR 1 drop (50 µl) pRBCs to Blue Top BLOOD PREP TUBE; cap tightly and gently invert several times to mix thoroughly. Place upright in test stand.
 PIPETTE 4 drops (200 µl) Recipient Serum or Plasma to Yellow Top REACTION TUBE.
From Blue Top BLOOD PREP TUBE, using a clean pipette for each transfer:
 TRANSFER 2 drops (100 µl) to Yellow Top REACTION TUBE. Replace cap, tighten and gently invert several times to mix thoroughly.
 TRANSFER 2 drops (100 µl) to Green Top NEGATIVE CONTROL tube. Replace cap, tighten and gently invert several times to mix thoroughly.
 TRANSFER 2 drops (100 µl) to Red Top POSITIVE CONTROL tube. Replace cap, tighten and gently invert several times to mix thoroughly.
 INCUBATE: Let all tubes stand for five (5) minutes at room temperature (20-25°C / 68-77°F).
 TRANSFER 1 drop (50 µl) from Yellow Top REACTION TUBE to Clear Top REACTION GEL tube (yellow-bordered labels). Cap tightly.
 TRANSFER 1 drop (50 µl) from Green Top NEGATIVE CONTROL tube to Clear Top NEGATIVE GEL tube (green-bordered labels). Cap tightly.
 TRANSFER 1 drop (50 µl) from Red Top POSITIVE CONTROL tube to Clear Top POSITIVE GEL tube (red-bordered labels). Cap tightly.
 PLACE Gel tubes in centrifuge and spin for a cumulative G force of 6,500. Refer to the included Crossmatch Centrifuge List for your model and spin setting.
If you do not have one of the listed centrifuges, refer to rapidvet.com under “Downloads” tab for a more complete centrifuge list for crossmatch; or call toll-free in US and Canada: (800) 567- 4367 or (908) 782-3353
Results Interpretation And Reporting
IMPORTANT: NEGATIVE GEL and POSITIVE GEL tubes serve as controls to ensure the test was run correctly. If gel controls do not react as stated below DO NOT proceed with the interpretation of test.
NEGATIVE GEL tube should demonstrate a collection of red blood cells at the bottom of the gel column.
POSITIVE GEL tube should demonstrate an agglutination of red blood cells at or near the top of the gel column (mid-matrix or above).
CROSSMATCH INTERPRETATION: If the REACTION GEL tube demonstrates a firm line of red blood cells at or near the top of the gel matrix, the reaction is POSITIVE and the Recipient is at risk for a transfusion reaction. DO NOT TRANSFUSE USING THIS DONOR.
If the vast majority of red blood cells are at or near the bottom of the gel matrix, the reaction is NEGATIVE. The Recipient is likely NOT at risk for demonstrating a transfusion reaction from this Donor at this time.
If the reaction shows a large number of cells suspended in the gel matrix without a firm line at the top, it is likely an indication of a minor incompatibility between the Recipient and this Donor. If no other more suitable donor is available, it is not likely that the Recipient is at risk for demonstrating a significant transfusion reaction from this Donor at this time.
Refer to the included Photo Identifier for examples of these reactions. Test results might be affected by the age of the cells used. Stored blood may exhibit a weaker reaction than that shown in the Photo Identifier. Record results using report card provided.
IMPORTANT NOTES: CROSSMATCHING IS DONE IN ADDITION TO, AND DOES NOT REPLACE, BLOOD TYPING.
Transfusions involving incompatible BLOOD TYPES will result in the activation of alloantibodies which may cause life-threatening reactions, or the production of antibodies which may cause serious complications in subsequent transfusions. In addition, the lifespan of incompatible RBCs will be shortened, increasing the need for further transfusions.
A compatible crossmatch does not prevent sensitization or delayed transfusion reactions in subsequent transfusions from the same donor. It simply indicates that at the present time there are no significant antibodies against the red cells.
If Oxyglobin® is in recipient blood, or in the event of severe hemolysis, this test is not recommended.
StorageShelf-life: 24 months. Store upright at room temperature until expiration date: DO NOT FREEZE.
DisposalDispose of all biological materials, pipettes and tubes in a biohazard container.
RapidVet is a registered trademark of dmslaboratories, inc.
Oxyglobin is a registered trademark of Biopure Corporation.
dmslaboratories, inc., 2 Darts Mill Road, Flemington, NJ 08822
(908) 782-3353 / 800 567-4367
Fax (908) 782-0832
Technical Services: 888-VET-TEST
2 DARTS MILL ROAD, FLEMINGTON, NJ, 08822
|Technical Service:||888-VET-TEST (838-8378)|
|Every effort has been made to ensure the accuracy of the RapidVet-H Companion Animal Major Crossmatch Test Kit information published above. However, it remains the responsibility of the readers to familiarize themselves with the product information contained on the US product label or package insert.|
Copyright © 2018 North American Compendiums. Updated: 2018-09-03