Enterovax

Tenosynovitis Vaccine

Modified Live Virus

DIRECTIONS FOR USE, READ CAREFULLY

ENTEROVAX Vaccine is a live vaccine containing an avian reovirus (tenosynovitis biotype) in a freeze-dried preparation sealed under vacuum. It is recommended for use in healthy chickens to aid in the prevention of reovirus induced tenosynovitis (viral arthritis) by spray or drinking water administration.

WHEN TO VACCINATE

SPRAY METHOD: 1 day of age.

DRINKING WATER: 1 week of age or older.

YOUR VACCINATION PROGRAM

The development of a durable, strong protection to this disease depends upon the use of an effective vaccination program as well as many circumstances such as administration techniques, environment and flock health at the time of vaccination. Also, the immune response to one vaccination under field conditions is seldom complete for all animals within a given flock. Even when vaccination is successful, the protection stimulated in individual animals against different diseases may not be life long. Therefore, a program of periodic revaccination may be necessary.

PREPARATION OF VACCINE

DO NOT OPEN AND MIX THE VACCINE UNTIL READY TO BEGIN VACCINATION. USE VACCINE IMMEDIATELY AFTER MIXING.

1. Remove the tear-off seal and stopper from the vial containing the lyophilized vaccine.

2. Carefully pour clean, cool, non-chlorinated tap water into the vaccine vial until the vial is approximately two-thirds full.

3. Insert the rubber stopper and shake vigorously until all material is dissolved.

4. The vaccine is now ready for drinking water or coarse spray use in accordance with the directions below. For best results, be sure to follow directions carefully.

HOW TO VACCINATE

BY SPRAY:

Use this method for one day of age vaccination. Proper coarse spray application of this vaccine is best accomplished through use of a clean spray cabinet adjusted to deliver approximately 7 mL per 100 chicks. Rehydrate the vaccine according to the above instructions “PREPARATION OF VACCINE.”

Further dilute each 1000 doses of vaccine to 70 mL, each 2500 doses of vaccine to 175 mL, or each 5000 doses to 350 mL using distilled water. Following the spray cabinet directions, adjust the cabinet so that 100 chicks are sprayed with 7 mL of the diluted vaccine over a two second period.

BY DRINKING WATER:

Do not mix the vaccine into the drinking water until ready for use. Drinking water for vaccination should be mixed with powdered milk to prevent inactivation from chlorine or other water additives and also to stabilize the vaccine virus. The powdered milk should be added to the water at the rate of 3 grams per 11 liters (one heaped teaspoon per 3 U.S. gallons or 2.5 Imp. gallons); or 90 grams per 300 liters (one heaped cupful per 80 U.S. gallons or 66 Imp. gallons).

Use only clean waterers and equipment free of disinfectants or sanitizers. All water must be withheld for at least 2 hours prior to vaccination to assure that all chickens drink. Mix the rehydrated vaccine in the quantity of drinking water (milk added) which will be consumed by thirsty chickens in approximately 1 hour.

Add the rehydrated vaccine to water as follows:

Distribute diluted vaccine so that all chickens are able to drink within one hour. The diluted vaccine should be the sole source of drinking water until completely consumed. Avoid placing water in direct sunlight.

CAUTION

1. FOR ANIMAL USE ONLY

2. Vaccinate only healthy chickens.

3. Do not spill or spatter the vaccine. Burn empty bottles, caps and all unused vaccine and accessories. Use entire contents of vial when first opened.

4. Wash hands thoroughly after using the vaccine.

5. Store at 2° to 7° C (35° to 45° F). Do not freeze.

6. Do not vaccinate within 21 days of slaughter.

7. Do not administer this product by injection. Studies have demonstrated that injection will produce significant mortality in day of age chickens.

8. Do not administer using an automatic eyedrop vaccinator (eg. Biojecter-Plus) to day of age chickens.

9. Do not administer this product to breeders 18 weeks of age or older. Studies have demonstrated that the vaccine virus will be shed in the eggs.

PRESERVATIVE

This vaccine contains gentamicin.

RECORDS

Keep a record of vaccine type, quantity, serial number, expiration date, and place of purchase; the date and time of vaccination; the number, age, breed, and location of the chickens; names of operators performing the vaccination and any observed reactions.

FEDERAL REGULATIONS PROHIBIT THE REPACKAGING OR SALE OF THE CONTENTS OF THIS CARTON IN FRACTIONAL UNITS. DO NOT ACCEPT IF SEAL IS BROKEN.

Intervet Inc., Merck Animal Health, division of Intervet Inc., Omaha, NE 68103 USA

U.S. Veterinary License No. 165A

1 800 211-3573 (USA)

1 866 683-7838 (CAN)

For patent information:

http://www.merck.com/product/patent/home.html

150499-01

CPN: 1047058.5