Vetscan Giardia Antigen Test Kit

2. Break the seal of the Extraction Buffer storage bulb located on the top of the Sample Dilution Device by bending the bulb back and forth until the internal blue stick breaks at its base. Squeeze the bulb 5 - 6 times to thoroughly mix the sample with the Extraction Buffer.

3. Remove a Test Device from the protective pouch and place it on a flat surface.

4. Using the buffer storage bulb and the sampling swab as a pipette, transfer 3 drops (~150µL) of the diluted sample into the round sample well of the Test Device. Hold the pipette vertically to ensure proper sample volume. Start timing.

5. Read the results at 10 minutes. Strong positive results may appear as soon as 1 minute, and weak positive results may take up to 10 minutes to appear. After 10 minutes, discard the Test Device because the results are no longer valid.

VII. INTERPRETATION OF RESULTS

Negative Results

The result is negative when one red to pink line appears in the C (control) area.

Positive Results

The result is positive when two red to pink lines appear. One red /pink line will appear in the T (test) area and one in the C (control) area. A red to pink line with any intensity in the T (test) area should be considered positive. Colored lines may be lighter or darker than each other.

Invalid Results

The test is invalid when no colored line appears in the C (control) area, even if a red to pink line appears in the T (test) area. Repeat the test using a new cassette. Colored lines that appear after 10 minutes are not diagnostic and should be ignored.

VIII. STORAGE

The test kit is to be stored at room temperature (15°-27°C, or 59°-80°F) for the duration of the shelf life. Avoid the test kit being subjected to excessive heat or freezing for a long period of time.

IX. SENSITIVITY AND SPECIFICITY

In an USDA approved study involving 243 samples, the Vetscan Giardia Rapid Test was compared to fecal wet mount microscopic assay of IFA. Western blots were used to resolve the discrepant results. The Vetscan Giardia Rapid Test correctly identified 106/108 positive canine fecal samples and 134/135 negative canine fecal samples. The sensitivity and specificity of the Vetscan Giardia Rapid Test were 98.1% and 99.3%, respectively.

X. REFERENCES

1. Kirkpatrick CE. Giardiasis. Vet Clin North Am Small Anim Pract. 1987, 17(6):1377-87.

2. Bowman DD, Lucio-Forster A. Cryptosporidiosis and giardiasis in dogs and cats: veterinary and public health importance. Exp. Parasitol. 2009, 124(1):121-7.

Manufacturer

SA Scientific, 4919 Golden Quail, San Antonio, TX 78240 USA

VLN/PCN 373/5G05.50

1-210-699-8800

Distributor

Zoetis Inc., 333 Portage St., Kalamazoo, MI 49007 USA

1-888-963-8471

www.zoetisus.com

Imported by

Zoetis Belgium S.A., Rue Laid Burniat 1, 1348 Louvain-La-Neuve Belgium

51591900

Presentation: 10 and 20 test kits.

CPN: 3690611.0