Gelmate (Canada)

WHEN TO TEST:

The Gelmate test is for the detection of acute and chronic inflammation in farm animals. The higher the concentration of inflammatory proteins, mainly gammaglobulin and fibrinogen, the quicker the formation of a solid clot when whole blood is added to the reagent. Each tube contains specially stabilized glutaraldehyde, EDTA, and a wetting agent.

DIRECTIONS:

A. WHOLE BLOOD

1. Add 1 ml whole blood to fill tube to blue line on Gelmate tube (fresh or EDTA blood).

2. Swirl tube to ensure mixing & note time.

3. Tilt tube at 2, 5 & 10 minutes - when blood no longer flows invert tube and gently tap - clot should remain firmly in place.

4. END POINT IS FORMATION OF A FIRM CLOT - IF THIS OCCURS IN:
<5 MIN there is significant inflammation
5-10 MIN there is moderate inflammation

Typical gammaglobulin and fibrinogen levels:

Clot <5 Minutes:

History of patient is important as inflammation within a few weeks of testing may result in a positive Gelmate even if problem has resolved.

Examples of conditions causing elevations in inflammatory proteins: pleurisy, internal abscesses, peritonitis, traumatic reticuloperitonitis

False readings can occur with severe anemia (excessive plasma) or severe dehydration (reduced plasma).

CAUTION:

The glutaraldehyde reagent is caustic, if skin or eye contact occurs wash with copious amount of water & consult physician.

DO NOT LEAVE TUBES IN HOT SUN

Recommend storage at 4°C

References

1. Bendixen, HJ. Nord. Vet Med. 1954, 6. 187-194

2. Liberg, P. Acta vet. Scand. 1973, 14. 712-722

3. Liberg, P. et al. Acta vet. Scand. 1975, 16. 236-243

4. Nansen, P. & K. Nielsen.Can J. comp. Med. 1966, 30. 327-331

5. Sandholm, M. Res. vet. Sci. 1974, 17. 32-35

6. Aslund, O. Nord. Vet Med. 1961, 13. 96-103

7. Nielsen, K. Dansk Vet. Tidsskr. 1975, 58. 652-655

8. Hansen, K. Dansk Vet. Tidsskr. 1985, 68. 151-156

PLASSVACC^®, 1535 Templeton Road, Templeton, CA 93465

800-654-9743

www.plasvaccusa.com

Presentation: 10 and 25 tests per kit.

CPN: 1253004.3