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Canine Red Blood Cells

This page contains information on Canine Red Blood Cells for veterinary use.
The information provided typically includes the following:
  • Canine Red Blood Cells Indications
  • Warnings and cautions for Canine Red Blood Cells
  • Direction and dosage information for Canine Red Blood Cells

Canine Red Blood Cells

This treatment applies to the following species:
Company: Animal Blood Resources International


The Package Insert is considered an extension of the blood and component container labels as the space on those labels is very limited.

No MSDS is required for these products. This Package Insert is supplied to conform with applicable government regulations. This document should be kept on file and readily available to personnel involved in the use of this product.

Blood and blood components are biologic products and, in the form of cellular products, living canine tissue intended for use in the treatment of canine patients. Professional judgment based on clinical evaluations determines the selection of components, dosage, the rate of administration and decisions in situations not covered in this general statement.

WARNING. In spite of serological testing, the risk of transmitting infectious agents to the patient is present. Careful donor selection, care, and available laboratory tests do not eliminate the hazard. Also, septic and toxic reactions can result from transfusion of bacterially contaminated blood and components. Such reactions are rare, but may be life threatening. In addition, blood components may contain certain immunizing substances other than those indicated on the label. For example, plasma may contain red blood cells and platelets as well as plasma. Therefore, this Package Insert as a whole or in part cannot be considered or interpreted as an expressed or implied warranty of the safety or fitness of the described blood or blood component when used for their intended purpose. Use of specific blood components as indicated by the individual patients clinical condition is needed to prevent inappropriate transfusion. Please note that whole blood is rarely considered an appropriate choice for transfusion. See the section Whole Blood - Indications, below.

Autologous transfusion techniques (such as intraoperative salvage and presurgical donation) should be considered whenever feasible in the perioperative setting, to reduce the risks of disease transmission and immune reactions from homologous donations.



Blood and components described in this Package Insert have been collected from canine donors which are maintained in isolated, controlled access colonies. The blood type of each donor is indicated on the product label. The colonies receive on site health care, and all animals are current on immunizations to include: Canine Distemper, Adenovirus type 2, Leptospirosis, Parainfluenza, Canine Parvo Virus, and Rabies.

Blood from donors maintained in controlled access colonies generally carries a lower risk of disease transmission than blood from volunteer donors.

Testing of Donor Blood

Testing of the donor’s blood is required before an animal is admitted into the colony as a donor and annually thereafter. All colony donors must test serologically or PCR negative for Canine brucellosis, Ehrlichia canis, Borrelia burgdorferi, Babesia, Anaplasmosis, Rickettsia rickettsia, Hepatozoon, Neorickettsia, Bartonella, hematotropic Mycoplasmas, Leishmania, and Dirofilaria immitis. The label on the container indicates whether the donor is DEA 1 negative, DEA 1 positive, or DEA 4 only.


Labels contain the following information:

1. The Name of the Blood Product.

2. Proper Temperature range for storage.

3. The minimum weight or volume.

4. Company name, address, telephone number and California Biologics registration number (if applicable).

5. The expiration date of the blood component.

6. The donor (serial) identification number.

7. Blood type of the donor.

8. Statement regarding this Package Insert.


The following general instructions pertain to Whole Blood and all the components described in this Package Insert.

● The intended recipient must be properly identified before the transfusion is started.

● The plastic blood container must not be vented.

● Blood and blood components must be administered through an appropriate blood filter. A blood administration set with standard (170-260 µ) clot filter is recommended when administering volumes greater than 50 mL. An 18 micron blood filter (HEMO-NATE® blood filter) is recommended for volumes less than 50 mL. Syringe filters not specifically produced and approved for blood should never be used for any product containing red cells.

● Before use, bags of blood or components should be gently agitated to thoroughly mix contents.

● No medications or solutions may be added to or infused through the same tubing with blood or components except 0.9% Sodium Chloride, Injection (USP).

● Lactated Ringer’s, Injection (USP) or other electrolyte solutions containing calcium should NEVER be added to or administered concurrently with blood or components collected in an anticoagulant containing citrate. All of the products described below contain and are collected into anticoagulants which contain citrate.

● Hemolysis may become evident during the storage of components containing red blood cells. Blood and components should be inspected for bacterial growth. If upon visual inspection the fitness of a component is questioned because of, for example, presence of hemolysis, a significant color change in the contents of the blood bag as compared to the tubing segments, floccular material, cloudy appearance or other problems, the component should not be used. Call Animal Blood Resources at 1-800-243-5759 for further evaluation. A slight pink tinge to the supernatant due to some free hemoglobin may be present and is acceptable for transfusion.

● When thawing frozen products in a water bath, care must be taken to prevent contamination of entry ports. The use of watertight protective plastic over wraps (such as ziplock bags) is recommended.

● Blood and components may be warmed to no more than 37° C during transfusion, if warming is clinically indicated for situations such as hypothermia, blood exchange or massive transfusions.

● Blood components have been prepared by techniques that aid in preserving sterility up to the time of expiration. If the container is entered in a fashion that could contaminate the contents of the container for any reason the component expires 4 hours after entry if maintained at room temperature (20° - 24° C), or 24 hours after entry if refrigerated (1° - 6° C).

● Unless otherwise indicated by the patient’s clinical condition, the rate should be slow, no greater than 0.11 mL / pound of body weight for the first 30 minutes of the transfusion. The patient should be observed during this period since some life threatening reactions occur after the infusion of only a small volume of incompatible blood. If a transfusion reaction occurs, the transfusion should be discontinued immediately and appropriate therapy initiated. The infusion should not be restarted.

● Completion of the transfusion should be prior to component expiration or within 4 hours of warming to room temperature, whichever is sooner. If blood or components cannot be infused in 4 hours, they should be divided into aliquots before reaching 6 degrees C and stored appropriately in the refrigerator until needed.

● A crossmatch should be conducted before every transfusion.

● The blood type of both the donor and the recipient should be known before transfusing whenever possible. When the blood type of the recipient is not known, only blood from type DEA 1 NEGATIVE donors should be given. First time transfusions with donors and recipients of unknown blood types should NEVER be considered safe.

● Blood transfusions should never be considered safe, even under optimum conditions, and should not be given unless there is no other acceptable treatment.



The component Red Blood Cells is prepared by centrifugal or gravitational separation of the red cells from the plasma. Red Blood Cells are collected in CPD or CP2D and stored on Adenine-Saline Solution to extend shelf life. A unit of Red Blood Cells on AS-3 solution has a PCV of 60 - 75% ±. This assumes a donor PCV of 40 - 55% ±. A Single Unit contains minimum 100 mL+ of Red Blood Cells in addition to 50 mL of AS, a Double Unit contains minimum 200 mL+ of Red Blood Cells in addition to 100mL of AS. This extra fluid volume should not be included when determining the volume of RBCs needed for transfusion, but must be considered as part of the patient’s total fluid requirement. Some platelets and or white blood cells may have been removed during processing and are nonviable due to refrigerated storage.

A-S solutions consist of glucose, adenine and sodium chloride in water for injection (USP). Additive Solutions formula one (AS-1) and five (AS-5) also contain mannitol as a red blood cell stabilizing agent. Adenine-Saline formula three (AS-3) contains additional citrate and phosphate and does not contain mannitol.

Red Blood Cell components may be prepared from Whole Blood collected in CPDA-1, CPD or CP2D, but unless otherwise noted, ABRI redcells are collected in CPD or CP2D. RBCs which are not suspended on A-S solution have the same shelf life as the whole blood from which they are removed: CPDA-1 provides 35 days, CPD and CP2D provides 21 days. Red Blood Cells suspended on Adenine-Saline solution have a shelf life of 42 days and are so identified on the label. The expiration date is indicated on the label.


This component increases the oxygen carrying capacity of the recipient’s blood by increasing the circulating red blood cell mass.

Canine Red Blood Cells Indications

Red Blood Cells is the component of choice for virtually all patients with a symptomatic deficit of oxygen carrying capacity. This component may be used to help restore blood volume following significant hypovolemia without significant red cell mass deficit.


Do not use Red Blood Cells when anemia can be corrected with specific medications. See “Whole Blood, Contraindications.”

Side Effects and Hazards

Side effects and hazards of Red Blood Cells (including disease transmission, bacteremia or endotoxemia, and hemolytic transfusion reactions) are similar to those for Whole Blood. However, the incidence of allergic reactions, circulatory overload and metabolic complications is lower because removing plasma reduces volume and the quantity of metabolites and antibodies. The risk of disease transmission remains the same.

If A-S solution containing mannitol is used, Red Blood Cells contain 0.375g of mannitol per Double (300 mL) unit. The amount of mannitol in approved additive solutions is far below that used to achieve a diuretic effect. It is unlikely that any side effects of the mannitol would be observed. Mannitol currently is not present in ABRI Red Cells.

Dosage and Administration

The dosage and administration of Red Blood Cells are similar to those for Whole Blood. (See “Whole Blood, Side Effects and Hazards, Circulatory Overload Reactions.”) Due to the addition of A-S solution to the red cells, additional dilution with 0.9% Sodium Chloride, Injection (USP) to improve flow is not needed. Do not add lactated ringers or any other fluids.


A reference list is available upon request from Animal Blood Bank Inc.

Animal Blood Resources International, PO Box 1118, Dixon CA 95620

(800) 243-5759

(707) 678-7350 PST and International

(517) 851-8244 EST and International

Summary Chart of Blood Components


Major Indications


Not Indicated For

Special Hazards

Rate of Infusion

Red Blood Cells

Symptomatic anemia

Restoration of oxygen carrying capacity

Pharmacologically treatable anemia, Coagulation deficiency

Infectious diseases; septic, toxic, allergic, febrile reactions; circulatory overload

As patient can tolerate but less than 4 hours per unit

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CPN: 1398001.4

P.O. BOX 609, STOCKBRIDGE, MI, 49285
Telephone:   800-2HELPK9 (800-243-5759)
Fax:   517-851-7762
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Copyright © 2022 Animalytix LLC. Updated: 2021-11-29