Alvedia Canine or Feline Lab Test BT

SAME MATERIAL PROVIDED FOR BOTH FELINE & CANINE LAB TEST

ADDITIONAL MATERIAL NEEDED:

- 20 CLEAN TEST TUBES (5ML)

- 1 PIPETTE WITH TIPS

OPTION: COMPLEMENTARY MATERIAL CAN BE PURCHASED FROM ALVEDIA

- 20 BLOOD COLLECTOR STRIPS

- 20 CLEAN TEST TUBES

10 µl of blood

2 minutes

Quick

Easy to use

Clean

Accurate

Archivable

LAB TEST BT PROCEDURE

TAKE 1 MEMBRANE OUT OF THE WHITE TUBE OR MORE IF YOU NEED TO BLOOD TYPE SEVERAL DOGS.

ADD 3 DROPS OF BUFFER INTO THE CLEAN TEST TUBE.

COLLECT 10µL OF WHOLE BLOOD WITH A PIPETTE.

ADD THE 10µL OF BLOOD INTO THE TUBE FILLED OF BUFFER AND MIX GENTLY.

INSERT THE MEMBRANE INTO THE WELL TO ALLOW THE MIGRATION.

WAIT FOR THE COMPLETE MIGRATION UNTIL THE CONTROL LINE BECOMES VISIBLE.

STICK THE MEMBRANE ON THE RESULT FORM TO INTERPRET THE RESULT.

EXAMPLE: IF YOU OBTAIN A LINE IN FRONT OF THE DEA 1, THE DOG IS DEA 1 POSITIVE.

RECORD THIS FORM.

LAB TEST BT RESULTS

THE DOG IS DEA 1 -

ABSENCE OF RED LINE IN FRONT OF «DEA 1» ARROW INDICATES A NEGATIVE REACTION.

THE DOG IS WEAK DEA 1 +

PRESENCE OF A WEAK RED LINE IN FRONT OF «DEA 1» INDICATES A POSITIVE REACTION.

THE DOG IS DEA 1 +

PRESENCE OF RED LINE IN FRONT OF «DEA 1» INDICATES A POSITIVE REACTION.

THE CAT IS A GROUP

PRESENCE OF RED LINE IN FRONT OF “A” ARROW INDICATES A POSITIVE REACTION FOR A GROUP.

THE CAT IS B GROUP

PRESENCE OF RED LINE IN FRONT OF “B” ARROW INDICATES A POSITIVE REACTION FOR B GROUP.

THE CAT IS AB GROUP:

PRESENCE OF 2 RED LINES IN FRONT OF “A” AND “B” ARROW INDICATES A POSITIVE REACTION FOR BOTH A AND B GROUP.

Importance Of Blood Typing

Canine

THE DETERMINATION OF DEA 1 ANTIGEN IS STRONGLY RECOMMENDED BEFORE ANY BLOOD TRANSFUSION TO AVOID A POTENT ALLOANTIBODY RESPONSE AGAINST THIS ANTIGEN AND TO AVOID ACUTE HAEMOLYTIC TRANSFUSION REACTION.

Feline

THE PRESENCE OF NATURALLY OCCURRING ALLO ANTIBODIES IN TYPE A AND IN TYPE B CATS REQUIRES THAT BLOOD TYPING BE PERFORMED PRIOR TO BLOOD TRANSFUSION TO AVOID ACUTE HAEMOLYTIC TRANSFUSION REACTION, AND IN BREEDINGS TO PREVENT NEONATAL ISOERYTHROLYSIS. BLOOD CAN BE TAKEN DIRECTLY FROM THE UMBILICAL CORD.

LAB TEST SCIENTIFIC INFORMATION

The Dea 1 Antigen

ALVEDIA’S SCIENTIFIC TEAM ESTABLISHED THAT DEA 1 ANTIGEN PRESENTS A LARGE RANGE OF EXPRESSION LEVEL ON THE RBC SURFACE BY USING FLOW CYTOMETER TECHNOLOGY. SIMILARLY TO THE RHESUS D MOLECULE IN HUMAN (NAMED WEAK D OR DU), SOME DOGS EXPRESS LOW LEVEL OF DE A1 ANTIGEN (CALLED WEAK DEA 1). THIS WORK SHOWS THAT THE WEAK DEA 1 WERE PREVIOUSLY TYPED AS DEA 1.2. A LARGE STUDY ON MORE THAN 500 DOGS SHOWS THAT OUR MEMBRANE TECHNOLOGY DETECTS PERFECTLY ALL RANGE OF DEA 1 ANTIGEN EXPRESSION FROM VERY STRONG TO VERY WEAK. CONSEQUENTLY, THE DEA 1 LINE WILL ALWAYS SHOW DIFFERENT INTENSITIES.

EXAMPLE OF DEA 1 BLOOD TYPING POSITIVE RESULTS WITH DIFFERENT INTENSITIES USING OUR LABTEST TECHNOLOGY

EXAMPLE OF DEA 1 BLOOD TYPING POSITIVE RESULTS WITH DIFFERENT INTENSITIES USING FLOW CYTOMETER ANALYSER

RELIABLE IN CASE OF AUTO-AGGLUTINATION

THANKS TO OUR SPECIFIC MEMBRANE TECHNOLOGY, THE AGGLUTINATED RED BLOOD CELLS WILL BE RETAINED AT THE BOTTOM OF THE MEMBRANE WHEREAS NON AGGLUTINATED RBC WILL CARRY ON MIGRATING UNTIL THE TOP OF THE MEMBRANE.

Reliable In Case Of Low Pcv (anemia)

THANKS TO THE SENSITIVITY OF OUR SPECIFIC MONOCLONAL ANTIBODIES, EVEN A LOW PCV WILL ALLOW YOU TO OBTAIN A RELIABLE BLOOD TYPING.

ALL YOU NEED FOR A SAFE TRANSFUSION

CONTACT US:

ALVEDIA, 11-13 RUE DES AULNES, 69760 LIMONEST - FRANCE

TEL: +33(0)478 380 239

FAX: +33(0)478 939 563

E-MAIL: CONTACT@ALVEDIAVET.COM

WWW.ALVEDIA.COM

CPN: 1442004.3