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This page contains information on Trovac-NDV for veterinary use.
The information provided typically includes the following:
  • Trovac-NDV Indications
  • Warnings and cautions for Trovac-NDV
  • Direction and dosage information for Trovac-NDV


This treatment applies to the following species:
Company: Boehringer Ingelheim Animal Health

Newcastle Disease-Fowl Pox Vaccine

Live Fowl Pox Vector

Trovac-NDV Indications

This product has been shown to be effective for the vaccination of healthy one-day-old chickens against Newcastle disease and Fowl Pox. The duration of immunity has not been established. For more information regarding efficacy and safety data go to


Administer subcutaneously only.

Do not mix with other products, except as specified on the label.

Use entire contents when first opened.

Do not vaccinate within 21 days before slaughter.

Penicillin and Streptomycin Sulfate are added as bacteriostatic agents.

Contains Amphotericin B as a fungistatic agent.

Inactivate unused contents before disposal.

Storage Conditions

AMPULES: Store in liquid nitrogen container.

DILUENT: Store at room temperature.

LIQUID NITROGEN CONTAINER: Carefully observe all liquid nitrogen precautions including wearing eye protection and gloves. Store in a cool, well-ventilated area. Check liquid nitrogen level daily. Keep container away from incubator intakes and chicken boxes. CAUTION: DO NOT HOLD AMPULE TOWARD FACE WHEN REMOVING FROM A LIQUID NITROGEN CONTAINER. NEVER REFREEZE A VACCINE AMPULE AFTER THAWING.


1. Dilute the vaccine only as directed, observing all precautions and warnings for handling.

2. Remove from the liquid nitrogen only the ampules that are going to be used immediately. Move quickly, but carefully.

3. Place the ampule(s) in a large, clean container of water at 68° to 86°F (20° to 30°C) to thaw ampule quickly. Thaw the entire contents. Gently swirl the ampule to disperse contents. Break ampule at its neck and quickly proceed as described below.

4. Use 200 mL of sterile diluent for each 1,000 doses of vaccine to be injected subcutaneously. Remove the cover from the diluent container. Draw contents of the ampule into a sterile 10 mL syringe fitted with an 18 to 20 gauge needle. Slowly add the contents of the vaccine ampule to the appropriate volume of diluent. Withdraw a small amount of the diluent, rinse the ampule once and add this to the vaccine-diluent mixture. Mix the contents of the diluent container thoroughly by swirling and inverting the container. Do not shake vigorously.

5. Keep the container of diluted vaccine in an ice bath. Use the vaccine-diluent mixture immediately. Agitate as needed to ensure a uniform suspension of the cells.

Use Of Vaccine For Subcutaneous Injection

IMPORTANT: Sterilize vaccinating equipment by autoclaving a minimum of 15 minutes at 250°F (121°C) or boiling in water for at least 20 minutes. Never allow chemical disinfectants to come in contact with vaccinating equipment.

1. Use a sterile automatic syringe with a 20 to 22 gauge 3/8” to 1/2” needle that is set to accurately deliver 0.2 mL per dose. Check the accuracy of delivery several times during the vaccination procedure.

2. Inject chickens under the loose skin at the back of the neck (subcutaneously), holding the chicken by the back of the neck just below the head. The loose skin in this area is raised by gently pinching with the thumb and forefinger. Insert the needle beneath the skin in a direction away from the head. Inject 0.2 mL per chicken. Avoid hitting the muscles and bones in the neck.

3. Use the entire contents of the vaccine container within one hour after mixing the vaccine with the diluent.

The birds should be vaccinated again with a live Newcastle disease vaccine at ten days of age or older to assure adequate protection.

Directions For Addition Of Blue Dye To Sterile Diluent

Blue dye may be aseptically added to sterile diluent using the following procedures:

1. Dye mixing chart:

Bag/bottle diluent size

200 mL
400 mL
600 mL
800 mL

1200 mL
1600 mL

1800 mL
2400 mL

Blue Dye to be added

0.5 mL

1.0 mL

1.5 mL

2. Swab top of rubber stoppers on dye vial and diluent container with alcohol; let dry.

3. Using a sterile syringe and needle, withdraw the contents of the vial.

4. Inject dye into diluent container and mix well.

5. Add vaccine according to the manufacturer’s recommendations.

6. Properly dispose of the dye container and any unused contents at the end of the day.

Liquid Nitrogen Precautions

The liquid nitrogen containers and vaccines should be handled only by properly trained personnel.

Liquid nitrogen is extremely cold. Accidental contact with skin or eyes can cause serious frostbite. Protect eyes with goggles or face shield. Wear gloves and long sleeves when removing and handling frozen ampules or when adding liquid nitrogen to the container.

Storage and handling of liquid nitrogen containers should be in a well-ventilated area. Excessive amounts of nitrogen reduce the concentration of oxygen in the air of an unventilated space and can cause asphyxiation. If drowsiness occurs, get fresh air quickly and ventilate the entire area. If a person becomes groggy or loses consciousness while working with liquid nitrogen, get the person to a well-ventilated area immediately. If breathing has stopped, begin artificial respiration. Call a physician immediately.


Do not vaccinate diseased birds.

Vaccinate all birds on the premises at one time.

Administer a full dose to each bird.

Avoid stress conditions during and following vaccination.

Do not place chickens in contaminated facilities.

Exposure to disease must be minimized as much as possible.

For veterinary use only.

In case of human exposure, contact a physician.


Boehringer Ingelheim’s vaccines have met the requirement of the USDA in regard to safety, purity, and potency. This vaccine has been tested by the Master Seed immunogenicity test for efficacy.

Trovac-NDV Caution

The capability of this vaccine to produce satisfactory results depends upon many factors including - but not limited to - conditions of storage and handling by the user, administration of the vaccine, health and responsiveness of individual chickens and degree of field exposure. Therefore, directions for use should be followed carefully. The use of this vaccine is subject to applicable local and federal laws and regulations.


Boehringer Ingelheim Animal Health USA Inc., Gainesville, GA 30501 USA

VLN/PCN 124/17C1.R0


Product Code RPNF9015



Presentation: 5 x 1,000 doses frozen.

CPN: 1105056.2

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Copyright © 2023 Animalytix LLC. Updated: 2023-11-27