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SNAP* 4Dx* Plus Test Kit (Canada)

This page contains information on SNAP* 4Dx* Plus Test Kit for veterinary use.
The information provided typically includes the following:
  • SNAP* 4Dx* Plus Test Kit Indications
  • Warnings and cautions for SNAP* 4Dx* Plus Test Kit
  • Direction and dosage information for SNAP* 4Dx* Plus Test Kit

SNAP* 4Dx* Plus Test Kit

This treatment applies to the following species:
Company: Idexx

Heartworm Antigen-Anaplasma-Borrelia Burgdorferi-Ehrlichia Antibody Test Kit

For veterinary use only.

SNAP* 4DX* Plus Test

In vitro diagnostics for the detection of Dirofilaria immitis antigen, antibody to Anaplasma phagocytophilum, antibody to Anaplasma platys, antibody to Borrelia burgdoferi, antibody to Ehrilichia canis, and antibody to Ehrlichia ewingii in canine serum, plasma, or whole blood.

Precautions and warnings

● All wastes should be properly decontaminated prior to disposal.

● Do not mix components from kits with different serial numbers.

● Do not use a SNAP device that has been activated prior to the addition of sample.

● Male-only heartworm infections typically produce antigen levels that are below the detection capability of this antigen test.

● Refer to country specific Material Safety Data Sheet for regional hazard identification.


● Store at 2-8°C until expiration date.

● SNAP devices and reagents can be stored at room temperature (18-25°C) for 6 months or until the expiration date, whichever occurs first.

● After SNAP devices and reagents are removed from 2-8°C for more than 24 hours, the expiration date is 6 months or the printed expiration date, whichever occurs first. If the 6 month expiration date occurs prior to the printed expiration date, record the new date in the space provided on the kit.

Kit components





1 or 5 bottles Anti-D. immitis/A. phagocytophilum/A. platys/B. burgdorferi/E. canis/E. ewingii: HRPO conjugate (preserved with ProClin™ 150)

7.0 mL


SNAP device

15 or 30

Reagents contained in each device:

Wash solution (preserved with ProClin™ 150)

0.4 mL

Substrate solution

0.6 mL

Other components: transfer pipettes, sample tubes, and reagent rack

Conjugate - H317/H412/P261/P280/P333+P313: May cause an allergic skin reaction. Harmful to aquatic life with long lasting effects. Avoid breathing mist/vapors. Wear protective gloves. If skin irritation or rash occurs: Get medical advice/attention.

Substrate Solution - H317/ P261/P280/ P302+P350/P333+P313: May cause an allergic skin reaction. Avoid breathing mist/vapors. Wear protective gloves. IF ON SKIN: Wash with plenty of soap and water. If skin irritation or rash occurs: Get medical advice/attention.

Wash Solution - H317/H319/H402/H412/P261/P280/P305+P351+P338/P337+P313/P333+P313: May cause an allergic skin reaction. Causes serious eye irritation. Harmful to aquatic life. Harmful to aquatic life with long lasting effects. Avoid breathing mist/vapors. Wear eye protection/face protection. Wear protective gloves. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. If eye irritation persists: Get medical advice/attention. If skin irritation or rash occurs: Get medical advice/attention.

Sample information

● Samples must be at room temperature (18-25°C) before beginning the test procedure.

● Serum, plasma, or anti-coagulated whole blood (eg. EDTA, heparin), either fresh or stored at 2-8°C for up to one week, can be used.

● For longer storage, serum, or plasma can be frozen (-20°C or colder) and then recentrifuged before use.

● Hemolyzed or lypemic samples will not affect results.

Test procedure

1. If stored in a refrigerator, allow all components to equilibrate at room temperature (18-25°C) for 30 minutes before use. Do not heat.

2. Using the pipette provided, dispense 3 drops of sample into a new sample tube.

3. Holding the bottle vertical, add 4 drops of conjugate to the sample tube.

4. Cap the sample tube and mix it thoroughly by inverting tube 3-5 times.

5. Place the device on a horizontal surface. Add the entire contents of the sample tube to the sample well, being careful not to splash the contents outside of the sample well.

The sample will flow across the result window, reaching the activation circle in 30-60 seconds. Some sample may remain in the sample well.

6. When color FIRST appears in the activation circle, push the activator firmly until it is flush with the device body.

Note: Some samples may not flow to the activation circle within 60 seconds, and, therefore, the circle may not turn color. In this case, press the activator button after the sample has flowed across the result window.

7. Read the test result at 8 minutes.

Note: The positive control may develop sooner, but results are not complete until 8 minutes.

Interpreting The Test Results

Positive Result

Any color development in the sample spots indicates the presence of Dirofilaria immitis antigen, A. phagocytophilum antibody, A. platys antibody, B. burgdorferi antibody, E. canis antibody, or E. ewingii antibody in the sample.


● The A. phagocytophilum/A. platys spot cannot differentiate between the two species: a positive result indicates presence of antibodies to A. phagocytophilum and/or A. platys.

● The E. canis/E. ewingii spot cannot differentiate between the two species: a positive result indicates presence of antibodies to E. canis and/or E. ewingii.

● In a low percentage of samples (0.027% as reported), interfering substances in the patient’s blood can cause all spots on the device to react as positive. In this event, the sample should be re-assayed as plasma or serum to reduce the likelihood of interference.

Negative Result

Only positive control spot develops color.

Invalid results

Background-If the sample is allowed to flow past the activation circle, background color may result. Some background color is normal. However, if colored background obscures test result, repeat the test.

No color development-If the positive control does not develop color, repeat the test.

Borrelia burgdorferi vaccine cross-reactivity-The Borrelia burgdorferi assay detects antibodies induced as a result of natural infection with the organism and not following immunization with the following vaccines:

Recombitek® Lyme, LymeVax®, and Galaxy® Lyme and Nobivac® Lyme.

Recombitek is a registered trademark of Merial, Inc. LymeVax is a registered trademark of Pfizer, Inc, Galaxy and Nobivac are registered trademarks of Merck Animal Health.

Symbol descriptions

IDEXX Technical Support

USA/Canada: 1 800 248 2483 •

Australia: 1300 44 33 99 •


VLN/PCN: 313/5P23.01

*SNAP and 4Dx are trademarks or registered trademarks of IDEXX Laboratories, Inc. or its affiliates in the United States and/or other countries.

Patent information:

© 2022 IDEXX Laboratories, Inc. All rights reserved.

IDEXX, One IDEXX Drive, Westbrook, Maine 04092 USA


CPN: 1243015.11

Technical Service:   800-248-2483
Telephone:   800-667-3411
Order Desk:   800-248-2483
Fax:   905-475-7609
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Copyright © 2023 Animalytix LLC. Updated: 2023-05-30