Alvedia Lab Test Canine XM

A NEGATIVE REACTION IN FRONT OF «XM» INDICATES THE ABSENCE OF CIRCULATING ANTIBODIES. THE DONOR BLOOD IS COMPATIBLE WITH THE RECIPIENT.

THE XM IS POSITIVE

A WEAK REACTION ATTEST OF A POSITIVE RESULT

A POSITIVE REACTION (+/- TO ++++) IN FRONT OF «XM» INDICATES THE PRESENCE OF ALLO ANTIBODIES OR CIRCULATING AUTO ANTIBODIES.

THE DONOR BLOOD IS INCOMPATIBLE WITH THE RECIPIENT.

LAB TEST XM MATERIAL

1 BOX OF LABTEST XM CONTAINS:

- 1 TUBE WITH 5 XM MEMBRANES (1 MEMBRANE = 1 CROSSMATCH)

- 1 WASH BUFFER

- 1 WHITE TOP BUFFER 1

- 1 BLUE TOP BUFFER 2

- 5 CLEAN TEST TUBES

- 5 BLOOD COLLECTORS STRIPS

- 5 SMALL PIPETTES (1ML) FOR SERUM

- 5 PIPETTES (3ML) FOR WASHING

- 5 RESULTS FORMS

ADDITIONAL MATERIAL NEEDED:

- 1 CENTRIFUGE

PACKAGING

BOX OF 5 CROSSMATCH TESTS

THE LABTEST XM BOX ALLOWS YOU TO PERFORM 5 CROSSMATCH TESTS.

(1 XM TEST = 1 RECIPIENT + 1 DONOR)

THE ENTIRE MATERIAL IS PROVIDED INTO THE BOX, YOU DO NOT NEED ANY REAGENTS.

THE ONLY MATERIAL NEEDED IS A CENTRIFUGE.

LAB TEST XM PROCEDURE (Major XM)

Between donor’s RBC & recipient’s plasma or serum

PREPARATION OF BLOOD SAMPLES:

- DONOR: USE RED BLOOD CELLS (EDTA/ACD/ CPD).

- RECIPIENT: CENTRIFUGE RECIPIENT BLOOD SAMPLE FOR PLASMA OR SERUM COLLECTION.

TAKE A CLEAN TEST TUBE AND ADD 3 DROPS OF BUFFER 1.

DIP THE EXTREMITY OF THE BLOOD COLLECTOR STRIP INTO THE DONOR TUBE (=10µL OF BLOOD).

MIX THE EXTREMITY OF THE BLOOD COLLECTOR STRIP INTO THE TUBE CONTAINING BUFFER 1.

TAKE THE SMALL PIPETTE TO COLLECT THE RECIPIENT’S SERUM OR PLASMA AND ADD 3 DROPS OF SERUM OR PLASMA INTO THE TUBE CONTAINING THE RBC SUSPENSION.

MIX GENTLY THE SUSPENSION AND INCUBATE AT ROOM TEMPERATURE DURING 10 MINUTES.

TAKE THE LARGER PIPETTE TO COLLECT THE WASH BUFFER AND ADD 3ML INTO YOUR SUSPENSION.

CENTRIFUGE THE TUBE FOR 1 MINUTE AT 500G OR 1600 RPM. DISCARD THE SUPERNATANT AND REPEAT STEP 7 & 8 TWO TIMES MORE.

ADD 2 DROPS OF BUFFER 2 ON THE PELLET AND MIX GENTLY

TAKE 1 XM MEMBRANE AND DIP IT INTO THE TUBE CONTAINING THE SUSPENSION TO ALLOW A COMPLETE MIGRATION.

STICK THE MEMBRANE ON THE RESULT FORM TO INTERPRET IF THE XM IS POSITIVE OR NEGATIVE AND RECORD THE FORM.

For minor crossmatch, perform the same procedure by taking serum or plasma from the donor with RBC of the recipient.

Importance Of Crossmatching

CROSSMATCHING AIMS TO ESTABLISH A SEROLOGICAL COMPATIBILITY BETWEEN THE RECIPIENT AND THE DONOR. THE CLASSICAL TECHNOLOGY USES AN AGGLUTINATION REACTION TO DETECT ALLO ANTIBODIES PRODUCED AFTER A PREVIOUS TRANSFUSION AND/OR THE NATURALLY ANTIBODIES.

OUR IMMUNO-CHROMATOGRAPHIC TECHNOLOGY WILL DETECT THE PRESENCE OF IMMUNOGLOBULIN AND/OR C3 COMPONENTS BINDING TO THE RED BLOOD CELLS SURFACE. IT INDICATES AN IN VITRO SENSITIZATION AND CAN THUS BE USED TO INDICATE THE PRESENCE OF ALLO ANTIBODIES IN PRE-TRANSFUSION COMPATIBILITY TESTING.

THIS TEST ALSO ALLOWS TO DETECT CIRCULATING AUTO ANTIBODIES IN THE RECIPIENT.

LAB TEST XM SCIENTIFIC DATA

CLINICAL CASES:

CASE N°1

Crossmatch between donor and recepient was done before transfusion. Crossmatch negative.

CASE N°2

Crossmatch between donor and recepient was done before transfusion. Crossmatch negative.

CASE N°3

THE WEAK DEA 1 PHENOTYPE HAS TO BE CONSIDERED AS A TRUE DEA 1 POSITIF BLOOD GROUP. EXPERIMENTAL DATA SHOW THAT THE WEAK DEA 1 PHENOTYPE INDUCES STRONG ANTI DEA 1 ALLO ANTIBODIES AFTER TRANSFUSION.

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