Ovucheck Rapid Well (Canada)

Bovine Milk Progesterone Qualitative Test Kit

Insert / Product #C004

2003-08-21

OVUCHECK^® RAPID WELL is used to measure the level of progesterone in a drop of cow’s milk. The quantity of progesterone present is indicated by a change in colour, which is compared with a standard.

OVUCHECK^® RAPID WELL is a simple and rapid test which provides a reliable aid to cow fertility management.

The amount of progesterone is almost zero at oestrus (heat) and then increases and remains at a high level. The progesterone level falls suddenly 17 to 20 days after the last heat, indicating the onset of the next cycle. This fall does not occur if the animal is pregnant.

Execution

A. Sample Collection

From collecting jar:

Whole milk samples can be taken from the collecting jar (any milking) into a clean container marked with the cow’s number.

Sampling by hand:

If sampling by hand from the udder, avoid foremilk by discarding the first five squirts from each quarter. Then collect an equal amount from each quarter into the container.

Samples not to be tested immediately can be stored for 24 hours in the refrigerator.

B. Test Procedures

- The kit’s components should be allowed to reach room temperature before testing begins (about 30 minutes).

- Milk samples should be allowed to reach room temperature before testing begins.

- Make sure every solution is properly mixed before use.

- No more than 7 milk samples should be tested with the standard on a single occasion.

A standard (S) is provided. It needs to be used each time an assay is performed.

1. Take out a strip of wells from the plastic bag.

2. Select the necessary number of wells (1 + n, n being the number of milk samples to test) by breaking the plastic between the wells. Put the unused wells back in the plastic bag. Mark for identification purposes the top of the first well you will use.

3. Add one drop of standard (S) using the dropper bottle to the first well and one drop of milk sample to the second well with a pipette kept vertically. Each milk sample to be tested shall be added to a different well. Always use a new pipette for each milk sample. Wells must either contain a standard or a milk sample, not both.

4. Keeping bottle A vertically, add 4 drops of reagent A to each well.

5. Cover the wells and let them stand for 5 minutes at room temperature.

6. Empty the contents of the wells into the sink and rinse gently the wells three times, using warm tap water. Dry by tapping onto absorbent paper.

7. Keeping bottle B vertically, add 2 drops of reagent B to each well.

8. Keeping bottle C vertically, add 3 drops of reagent C to each well.

9. Agitate the wells gently to mix the contents.

10. Place the wells on a sheet of white paper and observe from above. Three to five minutes after step 9, check that there is a blue colour in the standard well. If so, tap the wells to mix contents evenly and compare the colour in each sample well with that of the standard well.

Material

Reagents provided with the kit:

Precautions

- For in vitro veterinary diagnostic use only.

- Store the kit at 4 ± 2°C. NEVER FREEZE.

- Do not mix the reagents from different lot numbers.

- Do not use the kit after the expiry date indicated on the package.

- Bottles A and S contain a preservative. When emptying the wells contents into a sink, thoroughly flush away with excess volume of tap water.

- Keep out of the reach of children.

- Do not take by mouth.

- If eyes or skin are splashed, wash thoroughly with tap water.

- At the end of testing, return components to the refrigerator. Unused wells should be resealed in the plastic pouch. Used wells should be emptied, but not used again.

INTERPRETATION

Biovet Inc. 4375, Beaudry ave, St-Hyacinthe (Qc) J2S 8W2 Canada

Tél.: 1-888-8BIOVET Fax: (450) 771-2996 Technical services: support@biovet-inc.com www.biovet.ca

Presentation: 31 possible tests.

CPN: 36940051