Skip to Content

Alvedia Lab Test Canine XM (Canada)

This page contains information on Alvedia Lab Test Canine XM for veterinary use.
The information provided typically includes the following:
  • Alvedia Lab Test Canine XM Indications
  • Warnings and cautions for Alvedia Lab Test Canine XM
  • Direction and dosage information for Alvedia Lab Test Canine XM

Alvedia Lab Test Canine XM

This treatment applies to the following species:
Manufacturer: Canadian Animal Blood Bank

TECHNOLOGY

FOR CANINE CROSSMATCH TEST

THE FIRST XM IMMUNO-CHROMATOGRAPHY TECHNIQUE

WITH A SPECIFIC CANINE ANTI-GLOBULIN REAGENT

20 MINUTES PROCEDURE

- TIME SAVING

- EASY HANDLING

- RELIABLE RESULTS

- EASY INTERPRETATION

LAB TEST XM RESULTS

C = CONTROL LINE

THE XM IS NEGATIVE

A NEGATIVE REACTION IN FRONT OF «XM» INDICATES THE ABSENCE OF CIRCULATING ANTIBODIES. THE DONOR BLOOD IS COMPATIBLE WITH THE RECIPIENT.

THE XM IS POSITIVE

A WEAK REACTION ATTEST OF A POSITIVE RESULT

A POSITIVE REACTION (+/- TO ++++) IN FRONT OF «XM» INDICATES THE PRESENCE OF ALLO ANTIBODIES OR CIRCULATING AUTO ANTIBODIES.

THE DONOR BLOOD IS INCOMPATIBLE WITH THE RECIPIENT.

LAB TEST XM MATERIAL

1 BOX OF LABTEST XM CONTAINS:

- 1 TUBE WITH 5 XM MEMBRANES (1 MEMBRANE = 1 CROSSMATCH)

- 1 WASH BUFFER

- 1 WHITE TOP BUFFER 1

- 1 BLUE TOP BUFFER 2

- 5 CLEAN TEST TUBES

- 5 BLOOD COLLECTORS STRIPS

- 5 SMALL PIPETTES (1ML) FOR SERUM

- 5 PIPETTES (3ML) FOR WASHING

- 5 RESULTS FORMS

ADDITIONAL MATERIAL NEEDED:

- 1 CENTRIFUGE

Packaging

Box Of 5 Crossmatch Tests

THE LABTEST XM BOX ALLOWS YOU TO PERFORM 5 CROSSMATCH TESTS.

(1 XM TEST = 1 RECIPIENT + 1 DONOR)

THE ENTIRE MATERIAL IS PROVIDED INTO THE BOX, YOU DO NOT NEED ANY REAGENTS.

THE ONLY MATERIAL NEEDED IS A CENTRIFUGE.

LAB TEST XM PROCEDURE (Major XM)

Between donor’s RBC & recipient’s plasma or serum

PREPARATION OF BLOOD SAMPLES:

- DONOR: USE RED BLOOD CELLS (EDTA/ACD/ CPD).

- RECIPIENT: CENTRIFUGE RECIPIENT BLOOD SAMPLE FOR PLASMA OR SERUM COLLECTION.

TAKE A CLEAN TEST TUBE AND ADD 3 DROPS OF BUFFER 1.

DIP THE EXTREMITY OF THE BLOOD COLLECTOR STRIP INTO THE DONOR TUBE (=10µL OF BLOOD).

MIX THE EXTREMITY OF THE BLOOD COLLECTOR STRIP INTO THE TUBE CONTAINING BUFFER 1.

TAKE THE SMALL PIPETTE TO COLLECT THE RECIPIENT’S SERUM OR PLASMA AND ADD 3 DROPS OF SERUM OR PLASMA INTO THE TUBE CONTAINING THE RBC SUSPENSION.

MIX GENTLY THE SUSPENSION AND INCUBATE AT ROOM TEMPERATURE DURING 10 MINUTES.

TAKE THE LARGER PIPETTE TO COLLECT THE WASH BUFFER AND ADD 3ML INTO YOUR SUSPENSION.

CENTRIFUGE THE TUBE FOR 1 MINUTE AT 500G OR 1600 RPM. DISCARD THE SUPERNATANT AND REPEAT STEP 7 & 8 TWO TIMES MORE.

ADD 2 DROPS OF BUFFER 2 ON THE PELLET AND MIX GENTLY

TAKE 1 XM MEMBRANE AND DIP IT INTO THE TUBE CONTAINING THE SUSPENSION TO ALLOW A COMPLETE MIGRATION.

STICK THE MEMBRANE ON THE RESULT FORM TO INTERPRET IF THE XM IS POSITIVE OR NEGATIVE AND RECORD THE FORM.

For minor crossmatch, perform the same procedure by taking serum or plasma from the donor with RBC of the recipient.

Importance Of Crossmatching

CROSSMATCHING AIMS TO ESTABLISH A SEROLOGICAL COMPATIBILITY BETWEEN THE RECIPIENT AND THE DONOR. THE CLASSICAL TECHNOLOGY USES AN AGGLUTINATION REACTION TO DETECT ALLO ANTIBODIES PRODUCED AFTER A PREVIOUS TRANSFUSION AND/OR THE NATURALLY ANTIBODIES.

OUR IMMUNO-CHROMATOGRAPHIC TECHNOLOGY WILL DETECT THE PRESENCE OF IMMUNOGLOBULIN AND/OR C3 COMPONENTS BINDING TO THE RED BLOOD CELLS SURFACE. IT INDICATES AN IN VITRO SENSITIZATION AND CAN THUS BE USED TO INDICATE THE PRESENCE OF ALLO ANTIBODIES IN PRE-TRANSFUSION COMPATIBILITY TESTING.

THIS TEST ALSO ALLOWS TO DETECT CIRCULATING AUTO ANTIBODIES IN THE RECIPIENT.

LAB TEST XM SCIENTIFIC DATA

CLINICAL CASES:

CASE N°1

Crossmatch between donor and recepient was done before transfusion. Crossmatch negative.

CASE N°2

Crossmatch between donor and recepient was done before transfusion. Crossmatch negative.

CASE N°3

THE WEAK DEA 1 PHENOTYPE HAS TO BE CONSIDERED AS A TRUE DEA 1 POSITIF BLOOD GROUP. EXPERIMENTAL DATA SHOW THAT THE WEAK DEA 1 PHENOTYPE INDUCES STRONG ANTI DEA 1 ALLO ANTIBODIES AFTER TRANSFUSION.

The weak DEA 1 phenotype has to be considered as a true DEA 1 positif blood group. Experimental data show that the weak DEA 1 phenotype induces strong anti DEA 1 allo antibodies after transfusion.

CONTACT US:

TO ORDER

FOR PRICE LIST

FOR FURTHER INFORMATION

OR TO IDENTIFY YOUR LOCAL DISTRIBUTOR

Alvedia

Alice Veterinary Diagostic

11-13 RUE DES AULNES, 69760 LIMONEST - FRANCE

TEL: +33 478 380 239

FAX: +33 478 939 563

CONTACT@ALVEDIAVET.COM

WWW.ALVEDIA.COM

NAC No.: 1626003.0

CANADIAN ANIMAL BLOOD BANK
AB71 - 2055 NOTRE DAME AVENUE, WINNIPEG, MB, R3H 0J9
Telephone:   204-632-2586
Fax:   204-632-4859
Website:   www.canadiananimalbloodbank.ca
Every effort has been made to ensure the accuracy of the Alvedia Lab Test Canine XM information published above. However, it remains the responsibility of the readers to familiarize themselves with the product information contained on the Canadian product label or package insert.

Copyright © 2016 North American Compendiums. Updated: 2016-10-31

Hide