SUCCEED Equine Fecal Blood Test (Canada)

This page contains information on SUCCEED Equine Fecal Blood Test for veterinary use.
The information provided typically includes the following:

SUCCEED Equine Fecal Blood Test Indications
Warnings and cautions for SUCCEED Equine Fecal Blood Test
Direction and dosage information for SUCCEED Equine Fecal Blood Test

SUCCEED Equine Fecal Blood Test

This treatment applies to the following species:

Horses

Company: Freedom Health

RAPID DUAL ANTIBODY TEST DETECTS OCCULT EQUINE ALBUMIN AND HEMOGLOBIN IN A FECAL SAMPLE.

● Aids diagnosis of GI tract conditions in horses.

● Helps differentiate foregut and hindgut conditions.

FOR VETERINARY USE ONLY.

DESIGNED FOR STALL-SIDE USE.

Veterinarians should use this product stall-side to support a differential diagnosis of gastrointestinal health conditions in horses:

● To rule in or rule out the foregut vs. the hindgut as the primary location of a suspected condition.

● To contribute objective data to the process of diagnosing symptomatic horses.

● As part of regular check-ups for ongoing assessment of GI health and for early detection of potential pathological issues in asymptomatic horses.

● To assess response to treatment of GI tract conditions.

Refer to the technical information sheet inside the carton for more information.

Precautions

For in vitro diagnostic use. Do not use after expiration date printed on foil pack, visible through jar, or on back of test cassette. Store in original packaging. Keep refrigerated when not in use. Do not use if center of temperature indicator tag on jar is solid black. Do not use if center of temperature indicator tag on top of carton is solid black. Keep out of direct sunlight or heat sources.

For veterinary use only.

USE INSTRUCTIONS

Contains one complete SUCCEED^® equine fecal occult blood test, including:

● 1 clear plastic container

● 1 dual test cassette - contains both an albumin test (Test A) and a hemoglobin test (Test H)

● 1 disposable sample pipette

● 1 polyethylene glove

● 1 instruction sheet

● 1 desiccant pouch (for moisture)

PATIENT CONSIDERATIONS

1. Check horse for signs of external bleeding where blood may be introduced to the digestive tract, particularly around the mouth.

2. Avoid testing horses within 48 hours of racing, especially those prone to bleeding (i.e., exercise induced pulmonary hemorrhage) where horse may swallow blood.

3. Avoid testing horses within 48 hours of having teeth floated.

4. Avoid testing mares during ovulation or immediately post-partum, where bleeding may be present.

5. Avoid testing horses within five days following anthelmintic treatment.

Instructions

Failure to carefully follow these instructions may lead to incorrect interpretation.

RUNNING THE TEST

1. Remove all items from the plastic container and set aside. Do not discard container lid.

2. Using provided glove, carefully collect fecal material from a single, fresh, complete bowel movement of subject horse. (For most accurate results, feces should be as fresh as possible and no more than 30 minutes old.) Collect about a pinch from multiple locations in a complete fecal specimen. Do not palpate to obtain sample.

3. Drop each pinch-sized sample into the plastic container up to the Sample Fill Line (lower line) on the label. Allow fecal matter to sit loosely in the container - do not pack or compress.

Remove and discard glove before proceeding.

4. Add clean (potable) tap water to the fecal matter in the container. Fill to the Water Fill Line (top line). Do not use water from dirty containers or from sources that may be contaminated with equine blood. Water should be room temperature.

5. Replace cap on container, and shake vigorously to mix fecal matter and water. Set container on a flat surface, and remove lid.

6. Open foil pack and remove test cassette and sample pipette. (Discard empty foil pack and desiccant pouch.)

7. Use provided disposable pipette to collect solution from container. Be sure to collect liquid only, avoiding particles of fibrous material or silt. This can interfere with wicking of the sample into the test strips. For best results: (i) tip jar slightly, (ii) depress pipette bulb before placing in solution, (iii) insert tip of pipette below surface of solution and release bulb slowly to draw up solution.

8. Apply 2 drops of solution from pipette to EACH of the two sample wells. Depress pipette bulb slowly to produce large drops.

Watch for liquid to wick up the membrane in the test windows. Wait until red lines appear at the CTL point in each window, called “control lines.” Control lines on each strip are your assurance that the strips are functioning properly.

If wicking does not occur, check that well is not clogged with silt, and apply one more drop ONLY to non-wicking well. (Excess fluid can flood the test, negating the result.)

9. If a line appears at the TST point on either the Test A or Test H window, that is a positive test. Allow up to 15 minutes to assure a negative result. DO NOT read results after 15 minutes. Note that even a faint line reflects a positive test.

Reading The Tests

Observe results after applying the solution to each of the two sample wells on the cassette, per instructions.

1. If a Control line does not appear within 15 minutes (at the CTL point on the cassette) that test result is invalid. The test must then be repeated with a new SUCCEED Equine Fecal Blood Test.

2. If only a Control line appears on either strip, that test is NEGATIVE. NOTE: only a complete line is a meaningful result.

3. If both the Control and Test lines appear on either window (Test A or H), this indicates a POSITIVE test result. Even if the test line is faint, the test is POSITIVE.

4. Do not read test results after 15 minutes.

If you have questions about reading the SUCCEED Equine Fecal Blood Test, please call: 800-281-6727 from 8AM - 5PM ET Monday through Friday.

If a solid line appears only at the CTL point (Control line) on either Test A or Test H, this is a NEGATIVE result.

If a complete, solid line appears at the TST point (Test line) on either or both Test A and Test H, that is a POSITIVE test result. Test A detects equine albumin. Test H detects equine hemoglobin. Note - even if the test line is faint, the test is POSITIVE.

Interpreting The Results

The following chart summarizes the meaning of a positive and/or negative test results across SUCCEED FBT tests A and H.

		Albumin Test (Test A)
		Negative	Positive
Hemoglobin Test (Test H)	Negative	No GI tract blood components detected. Compromised basement membrane possible in foregut.	GI tract issue detected in hindgut. Compromised basement membrane possible in foregut.
Hemoglobin Test (Test H)	Positive	Active vascular bleeding in foregut.	GI tract issue detected in hindgut. Active vascular bleeding possible in foregut, hindgut or both.

NOTES: Test A uses antibodies for albumin, which typically breaks down in digestion, particularly when exposed to acids and digestive enzymes in the stomach and small intestine. Also, albumin may be present at the site of low-grade injury absent whole blood (i.e., albumin may be present without hemoglobin). Thus, a positive Test A with a negative Test H indicates low-grade lesions in the hindgut.

Test H uses antibodies for hemoglobin. Hemoglobin is more resistant to acids and enzymatic activity in the GI tract. Also, hemoglobin is only present with whole blood. Thus, a positive Test H, with negative Test A, will indicate bleeding from a foregut source. You will not obtain a positive result from either Test A or H when a horse has a foregut lesion equivalent to a grade 1 gastric ulcer, where whole blood loss does not occur.

For statistical data on the reliability of the FBT, refer to the Technical Information sheet in the outer 2-pack carton.

FREEDOM HEALTH, LLC, 65 Aurora Industrial Parkway, Aurora, OH 44202 USA

800-281-6727 • 24 hours/day

www.SucceedFBT.com

SUCCEED^® is a registered trademark, and Equine Fecal Blood Test™ is a trademark of Freedom Health LLC. © 2014. All Rights Reserved. U.S. Patent Nos. 7,629,180 and 8,168,446. Canadian Patent No. 2,601,368.

CONTENTS:

10 complete test kits. Each kit contains 1 dual test cassette, 1 sample pipette and 1 polyethylene glove, in plastic specimen jar with instruction sheet. (Desiccant pouch included to absorb moisture.)

50110

P510 Rev F 06/14

L501A Rev G 06/14

I501 Rev Ji 10/14

CPN: 1671002.2

FREEDOM HEALTH LLC
65 AURORA INDUSTRIAL PARKWAY, AURORA, OH, 44202-8088

Toll-Free:		800-281-6727
Website:		www.succeed-equine.com
		www.succeedFBT.com
		www.succeed-vet.com
Email:		info@succeed-equine.com

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