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SNAP* Giardia Antigen Test Kit (Canada)

This page contains information on SNAP* Giardia Antigen Test Kit for veterinary use.
The information provided typically includes the following:
  • SNAP* Giardia Antigen Test Kit Indications
  • Warnings and cautions for SNAP* Giardia Antigen Test Kit
  • Direction and dosage information for SNAP* Giardia Antigen Test Kit

SNAP* Giardia Antigen Test Kit

This treatment applies to the following species:
Manufacturer: Idexx

Giardia Antigen Test Kit

For veterinary use only.

SNAP* Giardia

The SNAP* Giardia Test Kit is a rapid enzyme immunoassay for detection of Giardia antigen in canine and feline feces. The presence of this antigen in fecal samples indicates the animal has ingested Giardia cysts, may be actively infected, and may be shedding cysts in feces. Symptoms include diarrhea, vomiting and anorexia, and infections are particularly dangerous for immuno-compromised animals.1,2

Precautions And Warnings

- Use a separate SNAP device and conjugate/swab device for each test. Do not reuse kit components.

- Infectious Giardia cysts may be shed in feces and remain viable for long periods. Handle all samples as if capable of transmitting infection.

- Properly dispose of contaminated materials and disinfect work areas.

- The bioactive spots on the SNAP device are dyed for quality control purposes. This dye washes out during the test and does not interfere with the test result or interpretation.

- The SNAP device must be in a horizontal position on a flat surface while the test is performed.

- Do not use a SNAP device that has been activated prior to the addition of a sample.

- Do not expose the SNAP device to extreme light after activation.

- Do not mix components from kits with different lot numbers.

- Do not use components past their expiration dates.

Storage

- SNAP devices and test reagents are stable until their expiration dates when stored at 2-8°C.

- All components must be at room temperature (18-25°C) before running the test. This may take up to 30 minutes, depending upon the temperature in your laboratory. Do not heat.

Kit Components

Item

Reagent

1.

15 conjugate/swabs

Each conjugate/swab contains 0.7 mL of anti-Giardia:peroxidase conjugate solution (contains gentamicin and Kathon as a preservative).

2.

15 SNAP Devices

Each SNAP device contains 0.4 mL of wash solution and 0.6 mL of substrate solution.

Sample Information

- Canine or feline feces can be used fresh, previously frozen, or stored at 2-8°C for up to seven days.

- Samples must be at room temperature (18-25°C) before beginning test procedure.

- The collection swab is not intended to be used rectally.

Test Procedure

1. Pull off the tube that covers the conjugate/swab device and coat the entire swab tip with a thin layer of fecal material. Replace the tube over the swab.

2. Break the plastic valve stem inside the reagent bulb by bending the assembly back-and-forth at the neck. Holding the device swab-tip-down, squeeze and release the bulb three times to pass the conjugate solution in the bulb to the swab tip.

3. Place the SNAP device on a flat surface. Remove the tube from the conjugate/swab device. Using the swab/bulb as a pipette, dispense 5 drops of the sample/conjugate solution into the sample well of the SNAP device, being careful not to splash the contents outside of the sample well.

The sample will flow across the result window, reaching the activation circle in approximately 30-60 seconds. Some sample may remain in the sample well.

When the sample FIRST appears in activation circle, push the activator button firmly until it is flush with the device body.

NOTE: Some samples may not flow to the activation circle within 60 seconds and, therefore, the circle may not turn color. In this case, press the activator button after the sample has flowed across the result window.

4. Wait 8 minutes, then read the test result.

NOTE: The positive control spot color may develop sooner, but test results are not complete until 8 minutes.

Interpreting Test Results

To determine the test result, read the reaction spots in the result window and compare the color intensity of the sample spot to that of the negative control spot.

Negative Result

The result is negative for a sample spot, if:

- There is no color on the sample spot and the negative control spot.

or

- Color on the sample spot is equal to the color on the negative control spot.

Positive Result

Giardia Ag

Color on the Giardia sample spot is darker than color on the negative control spot.

NOTE: Some positive results may have only light color intensity on the sample spot.

Invalid Result

The negative control spot serves as a safeguard against false-positives. Color development on the positive control spot indicates the test reagents are functional and helps indicate that the assay has been run properly.

- If the positive control spot does not develop color, the result is invalid. Repeat the test.

- If color on the negative control spot is darker than color on the sample spot, the test is invalid. Repeat the test.

- If invalid results are obtained for a sample when repeated, contact IDEXX Customer Support.

Sensitivity and Specificity of SNAP Giardia

Comparison Test

Sample Size
SNAP
Giardia Test/Reference Test

Sample Type

Relative Sensitivity and Specificity
95% Confidence Limit

Kappa Statistic

+/+

-/+

+/-

-/-

Total

Immunofluorescence microscopy

74

4

1

144

223

Fecal

Sen., 95% (95% CL 87%-98%)

Spec., 99% (95% CL 96%-100%)

0.95

Microplate ELISA

75

3

0

145

223

Fecal

Sen., 96% (95% CL 88%-99%)

Spec., 100% (95% CL 97%-100%)

0.97

CL = Confidence Limit

References

1. Lieb MS, Zajac AM. Giardiasis in dogs and cats, Veterinary Medicine. 1999; 94:793-802.

2. Hill SL, et al. Prevalence of enteric zoonotic organisms in cats. JAVMA. 2000;216(5):687-692.

For any questions or comments, or to report any unusual results, call IDEXX Customer Support:

USA/Canada 1 800 248 2483

Europe 00 800 1234 3399

Australia 1300 44 33 99

If possible, please have your kit lot number available.

Symbol Descriptions

U.S. Vet. License No. 313

Product Code 5G05.50

*SNAP is a trademark or a registered trademark of IDEXX Laboratories, Inc. or its affiliates in the United States and/or other countries.

Patent information: idexx.com/patents.

© 2012 IDEXX Laboratories, Inc.

IDEXX LABORATORIES, One IDEXX Drive, Westbrook, Maine 04092 USA

C/O IDEXX Canada, 3044 Bloor Street, Toronto, ON M8X 2Y8 Canada

idexx.com

06-11758-03

NAC No.: 12430104

IDEXX CANADA CORP.
1345 DENISON STREET, MARKHAM, ON, L3R 5V2
Technical Service:   800-248-2483
Telephone:   800-667-3411
Order Desk:   800-248-2483
Fax:   905-475-7609
Website:   www.idexx.ca
Email:   CustomerSupportCanada@idexx.com
Every effort has been made to ensure the accuracy of the SNAP* Giardia Antigen Test Kit information published above. However, it remains the responsibility of the readers to familiarize themselves with the product information contained on the Canadian product label or package insert.

Copyright © 2014 North American Compendiums. Updated: 2014-07-28

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