SNAP* cPL* Test Kit (Canada)

This page contains information on SNAP* cPL* Test Kit for veterinary use.
The information provided typically includes the following:

SNAP* cPL* Test Kit Indications
Warnings and cautions for SNAP* cPL* Test Kit
Direction and dosage information for SNAP* cPL* Test Kit

SNAP* cPL* Test Kit

This treatment applies to the following species:

Dogs

Company: Idexx

Canine Pancreatic Lipase Test Kit

For veterinary use only.

Canine SNAP* cPL*

In vitro test for the determination of pancreas-specific lipase levels in canine serum.

Precautions and warnings

● All wastes should be properly decontaminated prior to disposal.

● Do not mix components from kits with different lot numbers.

● Do not use a SNAP device that has been activated prior to the addition of sample.

● Refer to country specific Material Safety Data Sheet for regional hazard identification.

Storage

● Store at 2-8°C until expiration date.

Kit components

Item	Reagents	Quantity
1	1 bottle of anti-chicken:HRPO/anti-cPL: HRPO conjugate	4.0 mL
2	SNAP device	10
Each SNAP device contains 0.4 mL of wash solution and 0.6 mL of substrate solution.
Other components: transfer pipettes, sample tubes, and reagent rack

	Conjugate - H317/H402/H412/P261/P280/P333+P313: May cause an allergic skin reaction. Harmful to aquatic life. Harmful to aquatic life with long lasting effects. Avoid breathing mist/vapors. Wear protective gloves. If skin irritation or rash occurs: Get medical advice/attention.
	Substrate Solution - H317/ P261/P280/ P302+P350/P333+P313: May cause an allergic skin reaction. Avoid breathing mist/vapors. Wear protective gloves. IF ON SKIN: Wash with plenty of soap and water. If skin irritation or rash occurs: Get medical advice/attention.
	Wash Solution - H317/H319/H402/H412/P261/P280/P305+P351+P338/P337+P313/P333+P313: May cause an allergic skin reaction. Causes serious eye irritation. Harmful to aquatic life. Harmful to aquatic life with long lasting effects. Avoid breathing mist/vapors. Wear eye protection/face protection. Wear protective gloves. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. If eye irritation persists: Get medical advice/attention. If skin irritation or rash occurs: Get medical advice/attention.

Sample information

● Samples must be at room temperature (18-25°C) before beginning the test procedure.

● Serum, either fresh or stored at 2-8°C for up to one week, can be used.

● For longer storage, serum can be frozen (-20°C or colder) and then recentrifuged before use.

● Hemolyzed, icteric or lipemic samples will not affect test results.

Test procedure

1. Allow all components to equilibrate at room temperature (18-25°C) for at least 30 minutes. Do not heat.

2. Using the pipette provided, dispense 3 drops of sample into a new sample tube.

3. Holding the bottle vertically, add 4 drops of conjugate to the sample tube.

4. Cap the sample tube and mix thoroughly by inverting it 3-5 times.

5. Place the device on a horizontal surface. Add the entire contents of the sample tube to the sample well, being careful not to splash the contents outside of the sample well.

The sample will flow across the result window, reaching the activation circle in 30-60 seconds. Some sample may remain in the sample well.

6. When color FIRST appears in the activation circle, push the activator firmly until it is flush with the device body.

NOTE: Some samples may not flow to the activation circle within 60 seconds. In this case, press the activator after the sample has flowed across the result window.

7. Read test result 10 minutes from the time of activation.

Interpreting the test results

Abnormal Level

If the color intensity of the sample spot is equal to or darker than the color intensity of the reference spot, cPL levels are abnormal.

Normal Level

If the color intensity of the sample spot is lighter than the color intensity of the reference spot, cPL levels are normal.

NOTE: It is possible for no color to develop on the sample spot.

Invalid results

● Background-If the sample is allowed to flow past the activation circle, background color may result. Some background color is normal. However, if colored background obscures test result, repeat the test.

● No color development-If reference spot does not develop color, repeat the test.

Idexx Snapshot Dx* Analyzer

Test results can also be read using the SNAPshot Dx analyzer. A complete description of how to enter patient data and read test results using the SNAPshot Dx analyzer can be found in the SNAPshot Dx analyzer user guide.

Repeatability (qualitative Precision)

Repeatability studies were conducted by running multiple replicates of five canine serum pools on SNAP cPL devices. The canine pancreatic-specific lipase (cPL) concentration of each canine serum pool was measured using the Spec cPL assay. SNAP cPL devices were run and visually interpreted by a single operator and results were tabulated as either N (Normal) or A (Abnormal). Within-Day and Day-to-Day repeatability were determined by running n=10 replicates per pool over three days. The results of this study are guidelines.

cPL concentration (µg/L) as determined by Spec cPL	SNAP cPL repeatability
	Expected interpretation	Observed interpretation
	Expected interpretation	Day 1	Day 2	Day 3	Overall
52	N	10/10 N	10/10 N	10/10 N	30/30 N
120	N	10/10 N	10/10 N	10/10 N	30/30 N
337	A	10/10 A	10/10 A	9/10 A	29/30 A
561	A	10/10 A	10/10 A	10/10 A	30/30 A
1000	A	10/10 A	10/10 A	10/10 A	30/30 A

Agreement With Spec Cpl* Reference Method

A user group study was conducted to evaluate agreement between SNAP cPL visual results and Spec cPL quantitative results.

Study design:

● Twenty canine serum samples with known Spec cPL concentrations were assayed on SNAP cPL devices.

● Each device was visually interpreted twice by each of 14 veterinary professionals for a total of 28 observations per sample. (Total n=560)

● Veterinary professionals were blinded as to the Spec cPL concentrations of the samples.

Results:

96% (538) of the interpretations were correct.

Statistical significance:

With 95% confidence, there is 94.0-97.4% agreement between the SNAP cPL and Spec cPL methods.

Symbol descriptions

IDEXX Technical Support

USA/Canada: 1 800 248 2483 • idexx.com

Australia: 1300 44 33 99 • idexx.com.au

Europe: idexx.eu

*SNAP, SNAPshot Dx, Spec cPL and SNAP cPL are trademarks or registered trademarks of IDEXX Laboratories, Inc. or its affiliates in the United States and/or other countries.

Patent information: idexx.com/patents.

IDEXX, One IDEXX Drive, Westbrook, Maine 04092 USA

idexx.com

06-12090-09

CPN: 1243012.5

IDEXX LABORATORIES CANADA CORPORATION, IDEXX CANADA
1345 DENISON STREET, MARKHAM, ON, L3R 5V2

Technical Service:		800-248-2483
Telephone:		800-667-3411
Order Desk:		800-248-2483
Fax:		905-475-7609
Website:		www.idexx.ca
Email:		CAGInsideSalesAssociateCND@idexx.com

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