Gamma-Check-B

WHEN TO TEST:

Newborns ingesting good quality colostrum will have > 800 mg IgG/dl by 8 hr old. Testing calves as early as 8 hr allows oral supplementation with colostrum. However calves may be tested up to 48 hr to monitor the herd colostrum program.

DIRECTIONS:

A. WHOLE BLOOD

1. Add 1.5 ml to fill to black line on GAMMA-CHECK tube (fresh or EDTA blood).

2. Swirl tube to ensure mixing & note time.

3. Tilt tube every 2 minutes - when blood no longer flows invert tube and gently tap - clot should remain firmly in place.

4. END POINT IS FORMATION OF A FIRM CLOT - IF THIS OCCURS IN:
<5 MIN the IgG is above 800 mg/dl
5-10 MIN the IgG is 700-800 mg/dl

A jelly like clot that slips down the side is usually positive but should be retested using serum or a different type of test.

B. SERUM

1. Collect blood into a red top tube, allow to clot (30 min), then centrifuge to remove serum.

2. Repeat steps A 1-4 using serum.

On occasion a false negative can occur - we recommend retesting the calf with another test, such as the Radial Immunodiffusion test, prior to a plasma transfusion.

Sick calves (with elevated fibrinogen), dehydrated calves or hemolyzed samples may give false positive results. In these cases the Gamma-Check test should be run using serum or another test type.

CAUTION:

The glutaraldehyde reagent is caustic, if skin or eye contact occurs wash with copious amount of water & consult physician.

DO NOT LEAVE TUBES IN HOT SUN

Recommend storage at 4°C

References

1. Tennant, B et al. Use of the glutaraldehyde coagulation test for Detection of Hypogammaglobulinemia in Neonatal Calves JAVMA 174: 848-853 1979

2. Jones, D & Brook, D Evaluation of Gamma-Check-B as a Field Test for Immunoglobulin Determination in Calves. Agripractice 1995

PLASSVACC^®, 1535 Templeton Road, Templeton, CA 93465

800-654-9743

www.plasvaccusa.com

Presentation: 10 and 25 tests per kit.

CPN: 11280034