Dermatophytes (Canada)

In-House screening test for simple and fast identification of dermatophyte infections on dogs and cats

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v02 2021-09-07

DERMATOPHYTES is a fast, simple and easy kit to perform test, which enables the veterinarian to confirm the diagnosis of dermatophyte infections. Dermatophytosis, generally referred to as tinea or ringworm, is a cutaneous infection caused by different genera of fungi collectively called the ‘dermatophytes’. The main fungi responsible for dermatophytosis in domestic animals are Microsporum canis, Trichophyton mentagrophytes and Microsporum gypseum. Dermatophytosis is a zoonotic skin disease. Children, the elderly and immunocompromised people are special at-risk populations, but anyone in frequent contact with infected pets risks contracting the disease. Dermatophytosis is highly contagious. It is important to quickly identify infected animals in order to take appropriate control measures.

SPECIFICATIONS

● The kit is made of 10 upright glass bottles with screw-cap lids.

● Culture medium responds to colony growth of M. canis, M. gypseum, T. mentagrophytes, T. rubrum, T. tonsurans, E. floccosum and M. audouinii and prevents the growth of non-pathogenic saprophytic mycetes and bacteria.

● Positive results are indicated by a pH color change marker in the culture medium.

PRECAUTIONS

● For in vitro veterinary use only.

● Store at 2-8°C for a maximal conservation period. Do not freeze.

● Do not use the kit after the expiry date indicated on the package. Expiry date is based on a storage at 2-8°C.

● Use instruments which are STERILISED to manipulate the samples.

● All waste must be considered as infectious and be decontaminated before being discarded.

EXECUTION

1. Identify the flask by writing on it in indelible felt tip the name of the patient and the date.

2. Wash the lesion and surrounding area with soapy water without fungicide or disinfectant and dry with absorbent paper.

3. Carry out the sampling (fur, scab, skin, etc.) with haemostatic forceps or a lancet. Do not sample too much. Use instruments which are STERILISED.

4. Open the flask of culture medium, working if possible near a flame to avoid contamination.

5. Place the sample on the edge of the culture medium, against the inside surface of the flask. Do not put the sample in the medium.

6. Close the flask again without fully tightening the cap. Air exchanges are essential.

7. Incubate at room temperature (23 ± 2°C).

8. After 48 hours, the color of the culture medium begins to change if the sample contains dermatophytes: pink then clearly red. Leave the sample in position for about 10 to 14 days to identify the colonies.

Interpretation Of Results

DERMATOPHYTES: The change in color pink then clearly red appears with the development of fungal colonies which usually have a light pigment.

WARNING: Other pathogens can also cause a change of color in the culture medium. However, these can be distinguished from dermatophytes by the following characteristics: SAPROPHYTIC FUNGI: The development of colonies takes place well before the change of color. The colonies are dark. BACTERIA: Characteristic morphology of the colonies.

Biovet Inc., 4375 Beaudry ave, St-Hyacinthe (Qc) J2S 8W2 Canada

Tel.: 1-888-8BIOVET

Fax: (450) 771-4158

Technical Services: support@biovet-inc.com

Can. Vet. Biol. Estab. Lic. No 49

TRM-560

CPN: 3694006.2