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ZIPPTest Canine Giardia Lateral Flow Test Kit

This page contains information on ZIPPTest Canine Giardia Lateral Flow Test Kit for veterinary use.
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  • ZIPPTest Canine Giardia Lateral Flow Test Kit Indications
  • Warnings and cautions for ZIPPTest Canine Giardia Lateral Flow Test Kit
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ZIPPTest Canine Giardia Lateral Flow Test Kit

This treatment applies to the following species:
Manufacturer: Centaur

Giardia Antigen Test Kit

Disease Background Information

Giardia is a singled cell protozoa parasite that lives in the small intestines of animals, including humans and can be found in the United States and a number of other countries. Giardiasis, the infection caused by Giardia, is transmitted in a fecal oral route with the ingesting Giardia cysts. Upon ingestion, the Giardia cysts travel to the small intestine where the trophozoite, or active Giardia parasite, is released. The trophozoites adhere to the intestinal wall and reproduce by binary fission. In a percentage of these trophozoites, the organism encysts and is passed in the feces. This completes the life cycle and spreads the infection. The Giardia cysts in the feces can contaminate the environment through water or direct contact and ultimately infect other animals and people. Giardia infections are frequently asymptomatic however, when symptoms occur, it is typically with the presentation of acute, intermittent or chronic diarrhea. Infected animals are prone to weight loss due to malabsorption in the intestines. Asymptomatic or symptomatic infections are both detectable with immunological methods that detect the cyst wall protein shed in the feces.1

Test Principle

This test is a rapid (10 minute) assay based on the detection of Giardia specific antigens in canine fecal samples. The assay uses sensitized gold particles to bind up this antigen and deposit at the test line. The accumulation of this gold particle/antigen complex at the test line results in a band (line) that can be seen visually. A second control line indicates that the test has been performed correctly.

Kit Configurations

● Fifteen Test Kit

● Thirty Test Kit

Kit Contents

● Test Devices: 15 or 30 individually packaged test devices.

● Sample Dilution Buffer: 15 or 30 tubes containing 1 ml of sample dilution buffer with sodium azide as a preservative.

● Transfer Pipettes: 15 or 30

● Collection Swabs: 15 or 30

● Instruction for use: 1

Precautions

● Use the kit before the expiration date indicated on the kit box.

● Store the kit between 2-8 °C. Allow the kit to come to room temperature (15-30 °C) before using.

● Open the pouch containing the device immediately before use.

● Run the test on a flat, horizontal surface. Hold the sample pipette vertically when adding reagents to the sample window of the test device.

● Do not touch the white membrane surface at any time. This could tear the membrane resulting in an inaccurate test.

● Add only the correct amount of diluted fecal sample to the device.

● Dilution buffer contains sodium azide as a preservative.

● Do not concentrate samples.

Testing Procedure

1. Bring kit contents and samples to room temperature (15-30 °C).

2. Remove test device from foil pouch and place on a flat, horizontal surface.

3. Coat a thin layer of fecal sample on the tip of the collection swab, and vigorously mix into the sample dilution tube containing 1 ml of sample dilution buffer. Press the swab tip against the sides of the dilution tube to express as much fluid as possible.

4. Mix sample thoroughly by vortexing or using provided transfer pipette to pipette the diluted sample up and down.

5. Use the provided transfer pipette to add 4 drops or 200 µL of diluted fecal sample to the sample window on the test device. The same transfer pipette used to mix sample can be used to transfer diluted sample to the device sample window.

6. Set timer for 10 minutes after the addition of the diluted sample to the device sample window.

7. At the end of the 10 minutes, read the device visually.

Note: If no flow is observed, prepare the sample again with a thinner layer of fecal material and use a new device to rerun sample.

CAUTION: Do not deviate from the above procedure. Deviations will compromise test performance and results.

Reading Results

Hold sample at a comfortable distance from the eyes under adequate light so that the line(s) are clearly visible.

Interpretation of Results

Positive: Any visible line in the cassette window at both the test and the control line area indicates that Giardia specific antigens are present in the sample.

Negative: A line will be visible in the cassette window only in the control line area. This indicates that Giardia specific antigens are not present in the sample or are present at a concentration level below the detection limit of this assay.

Invalid Test: If no lines are visible in the cassette window OR if only the test line is visible the test is invalid. Any invalid test should be repeated.

References

1. Foster, R., Smith, M. (1997-2013). “Giardia (Giardia canis).” Peteducation.com.

430/PCN 5G05.01

For Animal Use Only

For Technical Service: Centaur, Inc.

Tel: (913) 390-6184

Fax: (913) 390-5907

Email: sales@centauranimalhealth.com

Website: www.centauranimalhealth.com

Manufactured by: SafePath Laboratories, LLC., Carlsbad, CA 92010 USA

Tel: (760) 929-7744

PN 4718 Rev. 1

CPN: 1488039.0

CENTAUR ANIMAL HEALTH
1351 - F WEST HWY 56, OLATHE, KS, 66061
Telephone:   913-390-6184
Order Desk:   800-236-6180
Fax:   913-390-5907
Website:   www.centauranimalhealth.com
Email:   sales@centauranimalhealth.com
Every effort has been made to ensure the accuracy of the ZIPPTest Canine Giardia Lateral Flow Test Kit information published above. However, it remains the responsibility of the readers to familiarize themselves with the product information contained on the US product label or package insert.

Copyright © 2018 Animalytix LLC. Updated: 2018-09-28

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