ZIPPTest Canine Giardia Lateral Flow Test KitThis page contains information on ZIPPTest Canine Giardia Lateral Flow Test Kit for veterinary use.
The information provided typically includes the following:
- ZIPPTest Canine Giardia Lateral Flow Test Kit Indications
- Warnings and cautions for ZIPPTest Canine Giardia Lateral Flow Test Kit
- Direction and dosage information for ZIPPTest Canine Giardia Lateral Flow Test Kit
ZIPPTest Canine Giardia Lateral Flow Test KitThis treatment applies to the following species:
Giardia Antigen Test Kit
Disease Background Information
Giardia is a singled cell protozoan parasite that lives in the small intestines of animals, including humans, cats and dogs. The parasite is distributed worldwide and is one of the most frequently identified sources of gastrointestinal infections.
Giardia is transmitted by ingestion of the cyst form of the organism. Once inside the host, the cyst breaks open and releases the trophozoite which replicates in the small intestines and produces the GI symptoms. While passing out of the small intestine, some of the trophozoites will encyst and be passed with the host’s feces. This completes the life cycle of the organism.
Giardia infections are frequently asymptomatic. When symptoms occur, it is typically with the presentation of acute, intermittent or chronic diarrhea. Infected animals are prone to weight loss due to malabsorption in the intestines. Since asymptomatic animals can spread the organism, it is important to identify and treat infected animals. Both asymptomatic and symptomatic infections are detectable with immunological methods that detect Giardia specific protein shed in the feces.1
This test is a rapid (10 minute) assay based on the detection of Giardia specific antigens in canine fecal samples. The assay uses sensitized gold particles to bind up this antigen and deposit at the test line. The accumulation of this gold particle/antigen complex at the test line results in a band (line) that can be seen visually. A second control line indicates that the test has been performed correctly.
● Fifteen Test Kit
● Thirty Test Kit
● Test Devices: 15 or 30 individually packaged test devices.
● Sample Dilution Buffer: 15 or 30 tubes containing 1 ml of sample dilution buffer with sodium azide as a preservative.
● Transfer Pipettes: 15 or 30
● Collection Swabs: 15 or 30
● Instruction for use: 1
● Use the kit before the expiration date indicated on the kit box.
● Store the kit between 2-8 °C. Allow the kit to come to room temperature (15-30 °C) before using.
● Open the pouch containing the device immediately before use.
● Run the test on a flat, horizontal surface. Hold the sample pipette vertically when adding reagents to the sample window of the test device.
● Do not touch the white membrane surface at any time. This could tear the membrane resulting in an inaccurate test.
● Add only the correct amount of diluted fecal sample to the device.
● Dilution buffer contains sodium azide as a preservative.
● Do not concentrate samples.
1. Bring kit contents and samples to room temperature (15-30 °C).
2. Remove test device from foil pouch and place on a flat, horizontal surface.
3. Coat a thin layer of fecal sample on the tip of the collection swab, and vigorously mix into the sample dilution tube containing 1 ml of sample dilution buffer. Press the swab tip against the sides of the dilution tube to express as much fluid as possible.
4. Mix sample thoroughly by vortexing or using provided transfer pipette to pipette the diluted sample up and down.
5. Use the provided transfer pipette to add 4 drops or 200 µL of diluted fecal sample to the sample window on the test device. The same transfer pipette used to mix sample can be used to transfer diluted sample to the device sample window.
6. Set timer for 10 minutes after the addition of the diluted sample to the device sample window.
7. At the end of the 10 minutes, read the device visually.
Note: If no flow is observed, prepare the sample again with a thinner layer of fecal material and use a new device to rerun sample.
CAUTION: Do not deviate from the above procedure. Deviations will compromise test performance and results.
Hold sample at a comfortable distance from the eyes under adequate light so that the line(s) are clearly visible.
Interpretation of Results
Positive: Any visible line in the cassette window at both the test and the control line area indicates that Giardia specific antigens are present in the sample.
Negative: A line will be visible in the cassette window only in the control line area. This indicates that Giardia specific antigens are not present in the sample or are present at a concentration level below the detection limit of this assay.
Invalid Test: If no lines are visible in the cassette window OR if only the test line is visible the test is invalid. Any invalid test should be repeated.
1. Bowman, Dwight D. 2003. Georgis’ Parasitology for Veterinarians, 8th Edition. St. Louis, MO, USA: Saunders.
VLN 430/PCN 5G05.01
For Animal Use Only
For Technical Service: Centaur, Inc.
Tel: (913) 390-6184
Fax: (913) 390-5907
Manufactured by: SafePath Laboratories, LLC., Carlsbad, CA 92010 USA
Tel: (760) 929-7744
PN 4718 Rev. 1
Centaur Animal Health and SafePath Laboratories are committed to quality. If you are not 100% satisfied with this product, contact us at (913) 390-6184, or firstname.lastname@example.org.
PN 4807 REV. 2
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