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Western blot analysis


Definition: a procedure in which proteins separated by electrophoresis in polyacrylamide gels are transferred (blotted) onto nitrocellulose or nylon membranes and identified by specific complexing with antibodies that are either pretagged or posttagged with a labeled secondary protein.

[coined to distinguish it from eponymic Southern blot a.]

See Also: immunoblot

Further information

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© Copyright 2018 Wolters Kluwer. All Rights Reserved. Review date: Sep 19, 2016.