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two-dimensional chromatography


Definition: paper chromatography in which a spot, located originally in one corner of a sheet, is developed in one direction along one side of the sheet, after which the sheet is rotated 90° and developed, with another solvent, in the new direction; the resultant spots are thus spread over the entire paper, producing a “map” or “fingerprint.” Also generalized to include chromatography followed by electrophoresis (or vice versa) and column chromatography followed by paper chromatography.

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© Copyright 2018 Wolters Kluwer. All Rights Reserved. Review date: Sep 19, 2016.