Definition: complete or “true” hemolysis observed in blood agar cultures of various bacteria, especially hemolytic streptococci and staphylococci; virtually all the erythrocytes are destroyed in a relatively wide, regularly circumscribed, circular zone about the colony, thereby resulting in a clear “halo” of transparent agar; the zone of hemolysis is frequently much wider than the diameter of the colony; the degree of change varies with species of erythrocytes.
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