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Rothera nitroprusside test

Pronunciation: roth'er-ah

Definition: a test for ketone bodies; 5 mL of fresh urine are saturated with solid ammonium sulfate and mixed with 10 drops of freshly prepared 2% sodium nitroprusside solution, which is then mixed with 10 drops of concentrated ammonia water and allowed to stand for 15 minutes; the presence of acetoacetic acid, or of larger concentrations of acetone, is indicated by the development of a blue-purple color.

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© Copyright 2017 Wolters Kluwer. All Rights Reserved. Review Date: Sep 19, 2016.
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