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plastic section stain

 

Definition:

  1. for electron microscopy, a stain (osmic acid, phosphotungistic acid, potassium permanganate) used on thin sections of plastic-embedded tissues, using differential attachment of heavy atoms to various cellular and tissue structures so that electrons will be absorbed and scattered by these structures to produce an image; to achieve differential staining, the stain must penetrate nonwettable plastic embedments;
  2. for light microscopy, a stain (alkaline toluidine blue, silver methenamine) used on plastic-embedded tissues to attain higher resolution and more detail than normally possible; semithick (0.5–1.5 mcm) sections are particularly useful in renal pathology, especially in combination with the phase microscope.
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© Copyright 2017 Wolters Kluwer. All Rights Reserved. Review Date: Sep 19, 2016.
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