Definition: a test for indican; solids in the urine are precipitated by means of a 20% solution of acetate of lead and then filtered, and to the filtrate is added fuming hydrochloric acid containing a small amount of ferric chloride solution; if indican is present, the addition of chloroform causes the formation of indigo, indicated by the blue color.
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Examples: glitazone, GI cocktail, etc.