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ligase chain reaction

 

Definition: a technique for target amplification of DNA in which DNA ligase is used to join two complementary oligonucleotide probes that have bound to a target sequence in vitro. The ligation product is used as a template for ligation of complementary oligonucleotides that, through repeated enzymatic processing, allow for logarithmic accumulation of products that can be used to determine the presence of the target of interest.

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© Copyright 2018 Wolters Kluwer. All Rights Reserved. Review date: Sep 19, 2016.

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