Gomori nonspecific alkaline phosphatase stain
Definition: a calcium-cobalt sulfide method using frozen sections or cold acetone- or formalin-fixed paraffin sections, plus sodium ß-glycerophosphate as a substrate at pH 9–9.5 with Mg2+ as activator; calcium ions precipitate the liberated phosphate, cobalt salt replaces the calcium phosphate, and ammonium sulfide converts the product to a black cobalt sulfide.
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