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fluorescent in situ hybridization

 

Definition: a method used to determine the chromosomal location or expression pattern of genomic DNA or cDNA fragments. The piece of DNA to be mapped (the “probe”) is labeled with a fluorescent dye and hybridized to a chromosome preparation or to a tissue section. The probe anneals to complementary DNA or RNA sequences. Examination of the chromosomes or tissue section under a fluorescence microscope reveals the number, size, and location of the target sequences.

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© Copyright 2018 Wolters Kluwer. All Rights Reserved. Review date: Sep 19, 2016.

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