Skip to Content

fluorescent antibody technique

 

Definition: a technique for antigen using a fluorescent antibody, usually performed by one of two methods: direct, immunoglobulin (antibody) conjugated with a fluorescent dye is added to tissue and combines with specific antigen (microbe or other), the resulting antigen-antibody complex located by fluorescence microscopy; or indirect, unlabeled immunoglobulin (antibody) is added to tissue and combines with specific antigen, after which the antigen-antibody complex is labeled with fluorescein-conjugated antiimmunoglobulin antibody with the resulting triple complex located by fluorescence microscopy.

Disclaimer: This site is designed to offer information for general educational purposes only. The health information furnished on this site and the interactive responses are not intended to be professional advice and are not intended to replace personal consultation with a qualified physician, pharmacist, or other healthcare professional. You must always seek the advice of a professional for questions related to a disease, disease symptoms, and appropriate therapeutic treatments.
© Copyright 2018 Wolters Kluwer. All Rights Reserved. Review Date: Sep 19, 2016.
Hide