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fluorescence microscopy


Definition: a procedure based on the fact that fluorescent materials emit visible light when they are irradiated with ultraviolet or violet-blue visible rays; some materials manifest this property naturally, whereas others may be treated with fluorescent solutions (somewhat analogous to staining); when the absorption of the specimen is in the relatively long ultraviolet range a filter that transmits these radiations is used, and a yellow filter is placed on or in the ocular; the background field is then dark, and any yellow or red fluorescence becomes visible.

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