Definition: A nuclease that releases one nucleotide at a time, serially, beginning at one end of a polynucleotide (nucleic acid); several have been prepared from Escherichia coli, designated exonuclease I, exonuclease II, etc.; exonuclease III, which removes nucleotides from 3' ends of DNA, is used in DNA sequencing.
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Examples: glitazone, GI cocktail, etc.