Definition: in immunology, a method for determination of association constants for hapten-antibody reactions in a system in which the hapten (dialyzable) and antibody (nondialyzable) solutions are separated by semipermeable membranes. Because, at equilibrium the quantity of free hapten will be the same in the two compartments, quantitative determinations can be made of hapten-bound antibody, free antibody, and free hapten.
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