Definition: the formation of biuret that gives a violet color as a result of the reaction of a polypeptide of more than three aminoacyl residues with CuSO4 in strongly alkaline solution; dipeptides and amino acids (except histidine, serine, and threonine) do not so react; used for the detection and quantification of polypeptides, or proteins, in biologic fluids.
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Examples: glitazone, GI cocktail, etc.
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