CytRx's Arimoclomol Demonstrates Statistically Significant Cellular Evidence of Both Neuroprotection and Neuroregeneration in Animal Stroke Study
- Breakthrough Results Provide Critical Insights into Arimoclomol's Mechanism of Action -
- Partnering Potential of Arimoclomol Further Strengthened -
LOS ANGELES--(BUSINESS WIRE)--Aug 24, 2009 - CytRx Corporation (NASDAQ: CYTR), a biopharmaceutical research and development company engaged in the development of high-value human therapeutics, today announced that study results demonstrated that arimoclomol exhibited both statistically significant neuroprotective and neuroregenerative effects in brain cells of animals induced with stroke, offering important insights into this drug candidate's mechanism of action. Orally administered arimoclomol is a molecular chaperone amplifier that is believed to help reduce the accumulation of damaged proteins that may play a role in multiple diseases and disorders.
“These results help explain how arimoclomol achieved the dramatic improvements in functional recovery that we observed in our previously announced rat stroke studies,” said CytRx President and CEO Steven A. Kriegsman. “This better understanding of arimoclomol's mechanism of action is an important piece of information that we expect will be valuable in attracting potential pharmaceutical or biotechnology partners for further development of this exciting drug candidate. Currently, t-PA is the only FDA-approved treatment for stroke and must be administered within three hours of the initiation of stroke. We believe that arimoclomol has the potential to reach a much larger market, as our animal studies have shown statistically significant results even when animals are treated ten hours or more after stroke onset. In addition, clinical testing of arimoclomol for stroke recovery could be less expensive, as more patients could be treated at fewer sites due to the expanded therapeutic window.”
The study was conducted under the direction of world-renowned stroke expert Dr. Michael Chopp of the Department of Neurology in the Henry Ford Health System, Detroit, Michigan and the Department of Physics, Oakland University, Rochester, Michigan. Detailed cellular analysis in the brains of rats treated with arimoclomol following stroke inducement showed significantly increased molecular chaperone expression, decreases in both the number of cells undergoing programmed cell death (apoptosis) and the number of microglial cells that cause inflammation, and, most importantly, increases in the number of developing neurons sprouting new projections, called neurites, which are indicative of neuroregeneration.
Stroke is the result of oxygen deprivation to regions of the brain through diminished blood flow, usually due to a blood clot. Stroke affects about 700,000 Americans each year. On average, every 40 seconds someone in the United States has a stroke, and stroke is the leading cause of serious, long-term disability, according to the American Heart Association.
In the study, stroke was induced in rats by introducing blood clots into the middle cerebral artery, causing cerebral oxygen deprivation. These stroke-induced rats in groups of 10 received an oral dose of arimoclomol or a control substance daily for 28 days with the initial dose administered either six, 10, 24 or 48 hours after stroke. Sections of brain tissue were then evaluated for each of the following: 1) chaperone protein HSP70; 2) the number of cells with the characteristic DNA cleavage caused by apoptosis; 3) the microglial cell marker IB4-receptor; or 4) a marker for newly developing brain cells called doublecortin. HSP70 and doublecortin were detected with specific antibodies to the respective proteins, DNA cleavage was detected by a standard assay called TUNEL, and IB4-receptor was measured by binding to IB4-ligand.
The results showed that arimoclomol treatment in all groups tended to increase the number of cells expressing HSP70 compared to controls in the zone surrounding the lesion, reaching statistical significance (p<0.05) in those animals initially dosed either six or 10 hours following the stroke event. Moreover, all arimoclomol-treated groups tended to have a reduction in the number of apoptotic cells and microglial cells compared to controls in the zone surrounding the lesion, again reaching statistical significance (p<0.05) in those animals initially treated either six or 10 hours after stroke. Importantly, treatment with arimoclomol initiated at all four time points following the stroke event significantly increased the number of newly developing neurons in both the zone surrounding the lesion, as well as in an area called the subventricular zone of the lateral ventricle – the typical origin of these cells prior to their migration to the damaged tissue.
“We expected arimoclomol to increase chaperone expression and therefore probably prevent cells from undergoing apoptosis; however, we were elated to discover that it also controlled the proliferation of these inflammatory microglial cells and stimulated neuroregeneration as well,” said Jack Barber, Ph.D., CytRx Chief Scientific Officer. “These latter effects may explain arimoclomol's ability to restore brain function even when administered well after the stroke event, because the negative effects of inflammatory cells and the positive effects of neuroregeneration occur much later than the initial damage.”
About Molecular Chaperone Regulation
CytRx is a leader in molecular chaperone regulation technology. The Company currently has two orally administered, clinical-stage drug candidates and recently discovered a series of additional compounds that may provide a pipeline for additional drug candidates. The Company's drug candidates are believed to function by regulating a normal cellular protein repair pathway through the activation or inhibition of "molecular chaperones." Because damaged proteins are thought to play a role in many diseases, activation of molecular chaperones that help to reduce the accumulation of misfolded proteins may have therapeutic efficacy in a broad range of disease states. Similarly, CytRx believes that the inhibition of molecular chaperones that normally help protect cancer cells from toxic misfolded proteins may result in the selective destruction of cancer cells.
About CytRx Corporation
CytRx Corporation is a biopharmaceutical research and development company engaged in the development of high-value human therapeutics. The CytRx drug development pipeline includes programs in clinical development for cancer indications, including tamibarotene in a registration study for the treatment of acute promyelocytic leukemia (APL). In addition, CytRx is developing two drug candidates based on its industry-leading molecular chaperone technology, which aims to repair or degrade misfolded proteins associated with disease. CytRx also maintains a 39% equity interest in publicly traded RXi Pharmaceuticals, Inc. (NASDAQ:RXII).
This press release contains forward-looking statements within the meaning of Section 21E of the Securities Exchange Act of 1934, as amended. Such statements involve risks and uncertainties that could cause actual events or results to differ materially from the events or results described in the forward-looking statements, including risks relating to the predictive power of this or any other animal model of stroke recovery to humans, the outcome or results of any future pre-clinical or clinical testing of arimoclomol for stroke recovery, uncertainties related to the impact of the FDA's clinical hold on our clinical program for arimoclomol for the treatment of amyotrophic lateral sclerosis on the timing and ability to initiate further clinical development for stroke recovery, the risk that any requirements imposed on our planned clinical trial design for stroke recovery by the FDA as a result of the concerns expressed in their clinical hold of our ALS program might adversely affect our ability to demonstrate that arimoclomol is efficacious in treating stroke patients, risks related to CytRx's ability to enter into partnerships to advance the clinical development of arimoclomol, risks related to CytRx's need for additional capital or strategic partnerships to fund its ongoing working capital needs and development efforts, risks related to the future market value of CytRx's investment in RXi and the liquidity of that investment, and the risks and uncertainties described in the most recent annual and quarterly reports filed by CytRx with the Securities and Exchange Commission and current reports filed since the date of CytRx's most recent annual report. All forward-looking statements are based upon information available to CytRx on the date the statements are first published. CytRx undertakes no obligation to publicly update or revise any forward-looking statements, whether as a result of new information, future events or otherwise.
Posted: August 2009