This is how the Tetanus Toxoid vaccine is made:
Clostridium tetani culture is grown in a peptone-based medium and detoxified
with formaldehyde. The detoxified material is then purified by serial ammonium sulfate fractionation, followed by sterile filtration, and the toxoid is adsorbed to aluminum potassium sulfate (alum). The adsorbed toxoid is diluted with physiological saline solution (0.85%) and thimerosal (a mercury derivative) is added to a final concentration of 1:10,000.
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