SNAP Canine Parvovirus Antigen Test Kit

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  • SNAP Canine Parvovirus Antigen Test Kit Indications
  • Warnings and cautions for SNAP Canine Parvovirus Antigen Test Kit
  • Direction and dosage information for SNAP Canine Parvovirus Antigen Test Kit

SNAP Canine Parvovirus Antigen Test Kit

This treatment applies to the following species:
Manufacturer: Idexx Labs.

Canine Parvovirus Antigen Test Kit

For veterinary use only.

SNAP* Parvo

The SNAP Canine Parvovirus Antigen Test Kit is a rapid enzyme immunoassay for the detection of canine parvovirus (CPV) antigen in canine feces. This test detects a surface protein antigen of CPV (including intact virus particles) shed in the feces of CPV-infected dogs.

Kit Components

Item

Reagent

1

5 swabs with anti-parvovirus: HRPO conjugate

2

5 SNAP devices

Each SNAP device contains 0.4 mL of wash solution and 0.6 mL of substrate solution.

Storage

- Store at 2-25°C until the expiration date.

- All components must be at room temperature (18-25°C) before running the test. Do not heat. This will take at least 30 minutes depending upon the temperature of your laboratory.

Precautions And Warnings

- Do not mix components from kits with different lot numbers.

- Properly dispose of contaminated materials and disinfect work areas.

- Do not use a SNAP device that has been activated prior to the addition of sample.

- The sampling swab is not designed to be used rectally.

- Lubricants may reduce test sensitivity by reducing the volume of feces tested.

Sample Information

- Samples must be at room temperature (18-25°C) before beginning the test procedure.

- Canine fecal matter is required for this test. Swabs are provided for sampling.

- Fecal samples can be stored at 2-8°C for 24 hours. If longer storage is required, samples should be frozen.

Test Procedure

1. If stored in a refrigerator, allow all components to equilibrate at room temperature (18-25°C) for 30 minutes. Do not heat.

2. Obtain a sampling swab and a SNAP device for each sample to be tested. Pull and twist the tube covering the swab tip to remove the tube from the swab/reagent bulb assembly (A). Using the swab, coat the swab tip with fecal material. Then, return the swab to the tube (B).

NOTE: Only a thin coat of fecal material on the swab is required; do not coat the swab with excess feces.

3. Break the purple valve stem inside the bulb assembly by bending the assembly at the narrow neck (C). (Rebending the opposite way may be helpful.) Squeeze the reagent bulb three times to pass the blue solution through the swab tip and mix it with the sample (D).

4. Place the SNAP device on a horizontal surface. Using the swab as a pipette, dispense 5 drops of the fluid into the sample well, being careful not to splash contents outside of the sample well (Figure 1).

The sample will flow across the result window, reaching the activation circle in 30-60 seconds. Some sample may remain in the sample well.

5. When color FIRST appears in the activation circle, push the activator firmly until it is flush with the device body (Figure 2).

NOTE: Some samples may not flow to the activation circle within 60 seconds, and, therefore, the circle may not turn color. In this case, press the activator after the sample has flowed across the result window.

6. Read the test result at 8 minutes.

Interpreting The Test Results

To determine the test result, read the reaction spots in the result window and compare the color intensity of the sample spot to that of the negative control spot.

Positive Results

Color development in the sample spots that is darker than the negative control indicates a positive result, and the presence of parvovirus antigen in the sample.

Negative Results

Color development only in the positive control spot indicates a negative result.

Invalid Results

- Negative control (safeguard against false-positives)-If color in the negative control spot is equal to or darker than the color in the sample spot, the result is invalid and the sample should be retested.

- No color development-If the positive control does not develop color, repeat the test.

- Background-If the sample is allowed to flow past the activation circle, background color may result. Some background color is normal. However, if colored background obscures the test result, repeat the test.

Sensitivity and Specificity

Comparison Test

Sample Size
Kit/ Reference

Sample Type

Relative Sensitivity and Specificity
95% Confidence Limit

Kappa Statistic

+/+

-/+

+/-

-/-

Total

Hemagglutination

48

0

0

25

73

Feces

Sen., 100% (95% CL 94%-100%)
Spec., 100% (95% CL 89%-100%)

1.0

Probe

126

0

2

103

231

Feces

Sen., 100% (95% CL 98%-100%)
Spec., 98% (95% CL 93%-99.8%)

0.98

CL = Confidence limit

The shed window for CPV is typically highest day 4 to day 7 post-infection and usually correlates with the onset of clinical signs. In puppies with moderate maternal antibody levels, viral shedding may be delayed by 1-2 days relative to the onset of clinical signs. Virus shedding begins to wane by day 8-10 (post infection). It is important, therefore, to collect feces for viral detection at the onset of clinical illness and if negative for CPV, retest in 1-2 days.1

Parvo Vaccine Cross-Reactivity-In a study of 64 dogs vaccinated with six different modified live CPV-2 vaccines, the SNAP Parvovirus Antigen Test Kit did not detect CPV-2 in their feces.

A population of 64 beagles with low or no antibody to canine parvovirus-2 (CPV-2) were vaccinated with one of five different combination vaccines (Duramune® Max 5, Ft. Dodge Animal Health; Progard® 5, Intervet; Vanguard® Plus 5L, Pfizer Animal Health; Recombitek® C4, Merial; Galaxy® DA 2PPv, Schering-Plough Animal Health) or one monovalent product (NeoPar® NEOTECH LLC) containing modified live CPV-2 vaccine. Fecal samples were collected on day 0 and on one or more of the following days: 3, 4, 5, 6 and 7 post-vaccination. All samples were tested for CPV-2 using the SNAP Parvovirus Antigen Test Kit. No cross-reactivity was detected.

Symbol Descriptions

IDEXX Technical Support

USA/Canada 1 800 248 2483

Europe 00800 1234 3399

Australia 1300 44 33 99

References

1. DeCaro, N. et al. “Maternally-derived antibodies in pups and protection from canine parvovirus infection.” Biologicals. 2005; (33): 261-267.

U.S. Vet. License No. 313

Product Code 5024.03

*SNAP is a trademark or registered trademark of IDEXX Laboratories, Inc. in the United States and/or other countries.

Patent information: idexx.com/patents.

© 2012 IDEXX Laboratories, Inc. All rights reserved.

IDEXX LABORATORIES, One IDEXX Drive, Westbrook, Maine 04092 USA

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IDEXX LABORATORIES, INC.
ONE IDEXX DRIVE, WESTBROOK, ME, 04092
Telephone:   207-556-0300
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Website:   www.idexx.com
Email:   lpdweb@idexx.com
Every effort has been made to ensure the accuracy of the SNAP Canine Parvovirus Antigen Test Kit information published above. However, it remains the responsibility of the readers to familiarize themselves with the product information contained on the US product label or package insert.

Copyright © 2014 North American Compendiums. Updated: 2014-04-11

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