SNAP 4Dx Test Kit

This page contains information on SNAP 4Dx Test Kit for veterinary use.
The information provided typically includes the following:
  • SNAP 4Dx Test Kit Indications
  • Warnings and cautions for SNAP 4Dx Test Kit
  • Direction and dosage information for SNAP 4Dx Test Kit

Snap 4dx Test Kit

This treatment applies to the following species:
Manufacturer: Idexx Labs.

canine Heartworm Antigen-anaplasma Phagocytophilum-borrelia Burgdorferi-ehrlichia Canis Antibody Test Kit

for Veterinary Use Only.

canine Snap* 4dx*

In vitro diagnostics for the detection of Dirofilaria immitis (HW) antigen, antibody to Anaplasma phagocytophilum (AP), antibody to Borrelia burgdoferi (LY) and antibody to Ehrilichia canis (EC) in canine serum, plasma or whole blood.

Precautions And Warnings

- All wastes should be properly decontaminated prior to disposal.

- Do not mix components from kits with different lot numbers.

- Do not use a SNAP device that has been activated prior to the addition of sample.

Storage

- Store at 2-8°C until expiration date.

- SNAP devices and reagents may be stored at room temperature (18-25°C) for 90 days or until printed expiration date, whichever occurs first.

- After SNAP devices and reagents are removed from 2-8°C for more than 24 hours, the expiration date is 90 days or the printed expiration date, whichever occurs first. If the 90-day expiration date occurs prior to the printed expiration date, record the new date in the space provided on the kit.

Kit Components

Item

Reagent

Volume

1

1 bottle anti-HW/AP/LY/EC:HRPO conjugate

7.0 mL

2

SNAP Device

5, 15 or 30

Each SNAP device contains 0.4 mL of wash solution and 0.6 mL of substrate solution.

Other Components

transfer pipettes, sample tubes, and reagent rack

Sample Information

- Samples must be at room temperature (18-25°C) before beginning the test procedure.

- Serum, plasma or anti-coagulated whole blood (eg. EDTA, heparin), either fresh or stored at 2-8°C for up to one week, can be used.

- For longer storage, serum or plasma can be frozen (-20°C or colder) and then recentrifuged before use.

- Hemolyzed or lypemic samples will not affect results.

Test Procedure

1. If Stored In A Refrigerator, Allow All Components To Equilibrate At Room Temperature (18-25°c) For 30 Minutes. do Not Heat.

2. Using The Pipette Provided, Dispense 3 Drops Of Sample into A New Sample Tube.

3. Holding The Bottle Vertical, Add 4 Drops Of Conjugate to The Sample Tube.

4. Cap The Sample Tube And Mix It Thoroughly By Inverting Tube 3-5 Times.

5. Place The Device On A Horizontal Surface. Add The Entire Contents Of The Sample Tube To The Sample Well, Being Careful Not To Splash The Contents Outside Of The Sample Well.

the Sample Will Flow Across The Result Window, Reaching The Activation Circle In Approximately 30-60 Seconds. Some Sample May Remain In The Sample Well.

6. When Color first Appears In The Activation Circle, Push The Activator Firmly Until It Is Flush With The Device Body.

note

Some samples may not flow to the activation circle within 60 seconds, and, therefore, the circle may not turn color. In this case, press the activator button after the sample has flowed across the result window.

7. Read the test result at eight minutes.

Interpreting the Test Results

Positive Result

Any color development in the sample spots indicates the presence of heartworm antigen, A. phagocytophilum antibody, B. burgdorferi antibody or E. canis antibody in the sample.

Note

Initial research data suggests the SNAP 4Dx Anaplasma phagocytophilum spot could cross react with Anaplasma platys. In studies involving dogs infected with a laboratory strain of A. platys, the SNAP 4Dx was reactive with serum from 10 out of 10 infected animals.

Negative Result

Only positive control spot develops color.

Invalid Results

- Background-if The Sample Is Allowed To Flow Past The Activation Circle, Background Color May Result. Some Background Color Is Normal. However, If Colored Background Obscures The Test Result, Repeat The Test.

- No Color Development-if The Positive Control Does Not Develop Color, Repeat The Test.

idexx Snapshot Dx* Analyzer

Test results can also be read using the SNAPshot Dx analyzer. A complete description of how to enter patient data and read test results using the SNAPshot Dx analyzer can be found in the SNAPshot Dx analyzer user guide.

Comparison Test

Sample Size
snap 4dx Test/reference Test

Sample Type

Relative Sensitivity And Specificity
95% Confidence Limit

Kappa Statistic

+/+

-/+

+/-

-/-

Total

HTWM1,2

118

1

0

236

355

Serum

Sen., 99.2% (95% CL 94.8%-100%)

Spec., 100% (95% CL 98%-100%)

0.99

A. phagocytophilum2

217

2

0

236

455

Serum

Sen., 99.1% (95% CL 96.5%-100%)

Spec., 100% (95% CL 98%-100%)

0.99

B. burgdorferi5

166

2

0

236

404

Serum

Sen., 98.8% (95% CL 95.4%-99.9%)

Spec., 100% (95% CL 98%-100%)

0.99

E. canis4

100

4

0

236

340

Serum

Sen., 96.2% (95% CL 90.1%-98.8%)

Spec., 100% (95% CL 98%-100%)

0.97

Reference 1Necropsy and 2PetChek 3A. phagocytophilum IFA / Western Blot4 E. canis IFA / Western Blot 5B. burgdorferi IFA / Western Blot

Sensitivity and Specificity are based on visual interpretation of SNAP results.

CL = Confidence limit

Symbol Descriptions

Idexx Technical Support

Usa/canada 1 800 248 2483

Europe 00800 1234 3399

Australia 1300 44 33 99

Idexx.com

U.S. Vet License No. 313

Product Code 5P20.00

*SNAP, SNAPshot Dx, 4Dx, and PetChek are trademarks or registered trademarks of IDEXX Laboratories, Inc. in the United States and/or other countries.

Covered by U.S. Patent Nos.: 5,627,026; 5,726,010; 5,726,013; 5,750,333; 6,007,999; 6,204,252; 6,306,402; 6,475,492; 6,660,274; 6,719,983; 6,740,744; 6,923,963; 6,964,855; 7,063,846; 7,087,372; 7,407,770; 7,439,321; 7,445,788, 7,449,191; 7,744,872 and/or 7,888,491. Other U.S. and/or foreign patents issued or pending.

© 2011 IDEXX Laboratories, Inc. All rights reserved.

06-05057-11

IDEXX LABORATORIES, One IDEXX Drive, Westbrook, Maine 04092 USA

Nac No.

11160663

IDEXX LABORATORIES, INC.
ONE IDEXX DRIVE, WESTBROOK, ME, 04092
Telephone:   207-556-0300
Fax:   207-556-4346
Pet Diagnostics/Order Desk:   800-248-2483
Poultry/Livestock Diagnostics:   800-548-9997
Poultry/Livestock Order Desk:   800-943-3999
Website:   www.idexx.com
Email:   webmaster@idexx.com
Every effort has been made to ensure the accuracy of the SNAP 4Dx Test Kit information published above. However, it remains the responsibility of the readers to familiarize themselves with the product information contained on the US product label or package insert.

Copyright © 2014 North American Compendiums. Updated: 2014-04-11

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