Newcastle-Bronchitis Vaccine (Conn. Type)

Manufacturer: Merial Select

Newcastle-Bronchitis Vaccine, B1 Type, B1 Strain, Conn. Type, Live Virus

U.s. Vet. Lic. No.

279

Active Ingredient(s)

These vaccines contain the freeze-dried B₁ strain of Newcastle disease virus and selected strains of infectious bronchitis virus of the Connecticut serotype. The virus has been propagated in fertile eggs from specific pathogen free flocks. The immunizing capability of these vaccines have been proven by a master seed immunogenicity test. The vaccine offers proven immunity with mild reactions.

Contains streptomycin and penicillin as bacteriostatic agents.

Contains fungizone as a fungistatic agent.

Notice: These vaccines have met the requirements of the USDA in regards to safety, purity, potency and the ability to immunize normal, susceptible chickens.

Newcastle-Bronchitis Vaccine (Conn. Type) Indications

These vaccines are recommended for the vaccination of healthy chickens as an aid in the prevention of Newcastle disease and infectious bronchitis, Connecticut serotype.

The frozen vaccine is recommended for use in healthy one day old chickens.

The freeze-dried vaccine is recommended for the vaccination of healthy one day old chickens using the coarse spray method or for the vaccination of healthy chickens 14 days of age or older administered either in the drinking water or by aerosol spray. The use of an aerosol spray is recommended for revaccination only.

Chickens to be vaccinated must be healthy and free of all diseases. It is essential that the chickens be maintained under good environmental conditions, and that exposure to disease viruses be reduced as much as possible in the field.

Newcastle-Bronchitis Vaccine (Conn. Type) Dosage And Administration

A. Frozen Vaccine: For one (1) day old chickens.

Preparation of the Vaccine for Use: Important: Sterilize the vaccinating equipment by autoclaving for a minimum of 15 minutes at 250°F (121°C) or by boiling in water for at least 20 minutes. Never allow chemical disinfectants to come into contact with the vaccinating equipment.

1. Remove only one (1) ampule of vaccine at a time from the liquid nitrogen container. Thaw and use immediately. Do not hold the ampule towards the face when removing it from a liquid nitrogen container. Never refreeze a vaccine ampule after thawing.

2. The contents of the ampule are thawed rapidly by immersing it in water at room temperature (15-25°C). Gently swirl the ampule to disperse the contents. Break the ampule at its neck and quickly proceed as described below.

3. Transfer the contents of the ampule into a sterile 2 mL or 5 mL syringe fitted with an 18 to 20 gauge needle to cool, distilled, de-ionized water (10,000 doses to 700 mL of water).

4. Use the vaccine mixture immediately as described below.

Method of Vaccination:

1. Use the coarse spray application method.

2. Attach the spray head to the container and set the nozzle at a coarse spray setting.

3. To each box of 100 chickens, administer the vaccine by spraying 18-24 inches above the box.

4. Each box of 100 chickens should receive approximately 7 mL of vaccine. The exact amount can be determined by spraying into a calibrated tube or container to arrive at the number of applications needed to deliver 7 mL of vaccine.

5. Whatever the volume of vaccine used, take care to administer 1,000 doses to 1,000 chickens.

B. Freeze-dried Vaccine: For one (1) day old chickens.

Directions for Coarse Spray Vaccination:

1. Be certain all vaccinating equipment is clean before use. Never allow chemical disinfectants to come into contact with the vaccinating equipment.

2. Do not open and mix the vaccine until ready to use.

3. Remove the aluminum seal and rubber stopper from vial.

4. Aseptically transfer 3-5 mL of cool, distilled water into vaccine vial and mix gently.

5. Transfer the dissolved vaccine into cool, distilled water; 350 mL for 5,000 doses. Mix thoroughly.

6. Attach the spray head to the container and adjust the nozzle for coarse spray.

7. Administer the vaccine by spraying 18-24 inches above each tray of chicks.

8. Each tray of 100 chickens should receive approximately 7 mL of vaccine. The exact amount can be determined by spraying into a calibrated tube to arrive at the number of applications necessary to deliver 7 mL of vaccine.

9. Whatever volume of vaccine is used in the spray, take care to administer 5,000 doses to 5,000 birds.

10. Since coarse spray application is of a large droplet size, aerosol-like mist of the vaccine is reduced. However, always wear goggles and a face mask while spraying a live virus vaccine.

11. Any equipment used for the application of a live virus vaccine should not be used for any other purpose.

Directions for Drinking Water Vaccination: Do not use the drinking water method for vaccination of chickens younger than two (2) weeks of age or less than satisfactory results may be obtained.

1. Do not open or mix the vaccine until ready to use.

2. Remove all medications, sanitizers and disinfectants from the drinking water 72 hours prior to vaccination. Clean and rinse waterers thoroughly.

3. Provide sufficient waterers so that all of the birds can drink at one time.

4. Withhold all water from the birds for two (2) to four (4) hours prior to vaccination to stimulate thirst.

5. Add nonfat dry milk to the water at the rate of 1 oz. per gallon before mixing the vaccine.

6. Remove the aluminum seal and rubber stopper from the vial.

7. Fill the vaccine vial with 3-5 mL of clean, cool water and mix gently.

8. Mix the dissolved vaccine with water as shown below:

Age of birds	Water per 5,000 doses vaccine
2-4 weeks	12.5 gallons (49.5 L)
4-8 weeks	25 gallons (94.5 L)
8 weeks or older	50 gallons (189.5 L)

9. Distribute the vaccine solution among the waterers. Avoid direct sunlight.

10. Do not provide any other drinking water until all of the vaccine solution has been consumed.

Directions for Aerosol Spray Vaccination: Do not use for initial vaccination. Use only for revaccination of healthy chickens two (2) weeks of age or older.

Use a sprayer delivering an aerosol mist to disperse the rehydrated vaccine quickly and evenly throughout the chicken house.

1. Prior to spraying, reduce the air flow to a minimum. Keep the air flow reduced for 20 minutes following spray vaccination.

2. Remove the aluminum seal from a vial of the vaccine.

3. Remove the rubber stopper and fill the vial with 3-5 mL of cool, distilled water.

4. Pour the rehydrated vaccine into a clean container and add 500 mL of cool, distilled water per 5,000 doses of vaccine. Mix thoroughly.

5. For aerosol spray vaccination, apply at the rate of 500 mL per 5,000 chickens.

6. Place the vaccine solution in the sprayer canister, set the discharge control and walk through the house spraying at the rate of 1,000 chickens per minute. Direct the spray above the heads of the birds.

7. Whatever volume of vaccine solution is used, take care to administer 5,000 doses of vaccine to 5,000 birds.

8. Avoid direct contact with the vaccine solution. Wear goggles and a face mask while spraying a live virus vaccine.

9. Any equipment used for the application of a live virus vaccine should not be used for any other purpose.

Precaution(s)

A. Storage Conditions for Frozen Vaccine:

Ampules: Store in a liquid nitrogen container.

Liquid nitrogen container: Carefully observe all liquid nitrogen precautions, including wearing eye protection and gloves. Store in a cool, well-ventilated area. Check the liquid nitrogen level once a day. Keep the container away from incubator intakes and chicken boxes.

Liquid Nitrogen Precautions: The liquid nitrogen containers and vaccines should be handled by properly trained personnel only. These persons should be familiar with the Union Carbide publication “Precautions and Safe Practices - Liquid Atmospheric Gases”, form #9888. Liquid nitrogen is extremely cold. Accidental contact with the skin or eyes can cause serious frostbite. Protect the eyes with goggles or a face shield. Wear gloves and long sleeves when removing and handling frozen ampules or when adding liquid nitrogen to the container. Storage and handling of liquid nitrogen containers should be in a well-ventilated area. Excessive amounts of nitrogen reduces the concentration of oxygen in the air of an unventilated space and can cause asphyxiation. If drowsiness occurs, get fresh air quickly and ventilate the entire area. If a person becomes groggy or loses consciousness while working with liquid nitrogen, get the person to a well-ventilated area immediately. If breathing has stopped, begin artificial respiration. Call a physician immediately.

B. Storage Conditions for Freeze-dried Vaccine:

Store the freeze-dried vaccine at 35-45°F (2-7°C). Do not freeze.

Newcastle-Bronchitis Vaccine (Conn. Type) Caution(s)

A. Frozen Vaccine:

Do not vaccinate diseased birds.

Vaccinate all of the birds on the premises at one time.

Administer a minimum of one dose for each bird.

Avoid stress conditions during and following the vaccination.

Do not place chickens on contaminated premises.

Exposure to disease must be minimized as much as possible.

For veterinary use only.

The capability of these vaccines to produce satisfactory results depends upon many factors, including, but not limited to, conditions of storage and handling by the user, administration of the vaccine, health and the responsiveness of individual animals and the degree of field exposure. Therefore, directions for use should be followed carefully.

The use of these vaccines is subject to applicable local and federal laws and regulations.

Use only in localities where permitted.

Newcastle disease virus can cause inflammation to the eyelids of humans. Care should be taken to avoid contamination of the eyes, hands and clothing with the vaccine.

Administer only as recommended.

Use the entire contents when first opened.

Burn the container and all unused contents.

B. Freeze-dried Vaccine:

Do not vaccinate diseased chickens.

Birds should be free of respiratory disease and parasite infestations.

Vaccinate all of the birds on the same premises at the same time.

Administer 5,000 doses to 5,000 chickens.

Avoid stress conditions during and following the vaccination.

Do not place chickens on contaminated premises.

Exposure to disease must be minimized as much as possible.

Do not start the vaccination program for replacement pullets after birds are 16 weeks old.

The vaccine is designed to stimulate effective immunity when used as directed, but the user must be advised that the response to the product depends upon many factors, including, but not limited to, conditions of storage and handling by the user, administration of the vaccine, health and the responsiveness of individual birds, and the degree of field exposure. Therefore, directions for use should be followed carefully.

The product must be stored, transported, and administered in accordance with instructions and directions.

The use of the vaccine is subject to state laws wherever applicable.

Use only in states where permitted.

Use the entire contents when first opened.

Burn the container and all unused contents.

Since Newcastle disease virus can cause inflammation to the eyelids of humans, care should be taken to avoid contamination of the eyes, hands and clothing with the vaccine.

Warning(s)

Do not vaccinate within 21 days before slaughter.

Presentation

Frozen vaccine: 5 x 10,000 dose vials.
Freeze-dried vaccine: 25 x 5,000 dose vials.

Nac No.

11050361