Mycoplasma Meleagridis Nobilis Antigen Test KitThis page contains information on Mycoplasma Meleagridis Nobilis Antigen Test Kit for veterinary use.
The information provided typically includes the following:
- Mycoplasma Meleagridis Nobilis Antigen Test Kit Indications
- Warnings and cautions for Mycoplasma Meleagridis Nobilis Antigen Test Kit
- Direction and dosage information for Mycoplasma Meleagridis Nobilis Antigen Test Kit
Mycoplasma Meleagridis Nobilis Antigen Test KitThis treatment applies to the following species:
H-serotype, Plate Stained Antigen For Plate Agglutination Test
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This antigen is a suspension of killed, stained Yamamoto 529 Strain Mycoplasma meleagridis (MM) antigen for serum plate testing of turkey serum. The reactivity has been standardized against a reference serum.
Mycoplasma Meleagridis Nobilis Antigen Test Kit Indications
The antigen is used for the detection of MM antibody in turkeys. It is possible that not all birds in an infected flock will show a positive reaction, thus it is strongly recommended to use the serum plate agglutination (SPA) test for flock diagnosis and not for the individual identification of infected birds.
MM infection can be subtly present in a flock. It is therefore recommended only to consider a flock as negative when the test has been repeated several times and has given a negative result each time. Before officially designating a flock as being infected or not infected, consult your poultry pathologist. Plate testing is a screening method only. Do not destroy a flock based upon results of testing with this antigen.
Performance Of The Test
This serum plate MM test should be considered a basic screening test for antibodies. Under normal circumstances, the rate of nonspecific false positive reactions is low. However, nonspecific reactions may occasionally be high for a variety of reasons.
The hemagglutination inhibition (HI) test is too cumbersome for routine screening use. Positive HI reactions are generally accurate however, and are useful in evaluating serum samples that react with the plate antigen. The test should be conducted with four HA units. Titers of 1:80 or greater for turkey sera are considered positive, while a 1:40 titer would be suspicious and additional tests are required. Titers of 1:20 or less are considered negative.
The testing sequence for flock should be as follows:
1. Conduct SPA test using Intervet Antigen.
2. Test positive SPA serum samples with HI antigen.
3. Utilize HI results, additional tests (ie. culture, etc.) and your local poultry pathologist before determining Mycoplasma status of a flock. Always refer to the NPIP manual as mentioned in this sheet.
The serum plate test for MM is conducted by contacting and mixing 0.05 ml of test serum with 0.05 ml of serum plate antigen on a glass plate at room temperature. The standard procedure is:
1. DO NOT DILUTE THIS ANTIGEN.
2. Allow antigen and test serums to warm up to room temperature before use. Do not allow serum to be frozen prior to plate testing.
3. Dispense test sera in 0.05 ml amounts with a pipette (rinsed between samples) to 1 1/2” squares on a ruled glass plate. Limit the number of samples (no more than 25) to be set up at one time according to the speed of the operator. Serum should not dry out before being mixed with antigen.
4. Do not dilute serum.
5. Shake antigen prior to use. Dispense 0.05 ml of antigen beside the test serum on each square. Hold antigen dispensing dropper vertically.
6. Mix the serum and antigen, using a multimixing device if large numbers are to be run at one time.
7. Rotate the plate for 5 seconds. At the end of the first minute, rotate the plate again for 5 seconds and read 55 seconds later.
8. A positive reaction is characterized by the formation of definite clumps, usually starting at the periphery of the mixture. Most samples that are positive will react within the two-minute test period. Whenever samples are run, the antigen should be tested against known positive and negative control serums.
9. Some reactions may occur after two minutes, these should be considered suspect.
For additional information on Mycoplasma test procedures, refer to the following references: Proc. 77th Annual Meeting, U.S. Animal Health Association, 1973; Isolation and Identification of Avian Pathogens, 2nd Edition; Methods for Examining Poultry Biologics and for Identifying and Quantifying Avian Pathogens, 1971; and the National Poultry Improvement Plan and Auxiliary Provisions Manual; USDA-APHIS, June 1985.
This product is not hazardous when used according to directions supplied. A material safety data sheet (MSDS) is available upon request. This and any other consumer information can be obtained by calling Intervet Customer Service at 1-800-441-8272 or 1-302-934-8051.
Ten ml of antigen is packaged in a glass bottle fitted with a dropper top. This top is calibrated to deliver 0.05 ml of antigen per test. The antigen should be stored in a dark area at 2-7°C (35-45°F).
10 mL - 200 Tests
INTERVET INC., Millsboro, DE 19966
U.S. Veterinary License No. 165A
Made in U.S.A.
556 MORRIS AVE., SUMMIT, NJ, 07901
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Copyright © 2014 North American Compendiums. Updated: 2014-09-05