Gelmate (Canada)

Manufacturer: V.D.I.

Contents

Each tube contains stabilized glutaraldehyde, EDTA, a wetting agent and a partial vacuum.

Gelmate Indications

GELMATE is used for the detection of acute or chronic inflammation in horses and farm animals.

Test Principle

The GELMATE test is for the detection of acute and chronic inflammation in horses and farm animals. The test works on the principle that glutaraldehyde reacts with gammaglobulin (GG) and fibrinogen in whole blood to form a solid gel. The higher the concentrations of these proteins, the quicker the reaction occurs. Each tube contains stabilized glutaraldehyde, EDTA, a wetting agent and a partial vacuum.

Test Procedure

To perform the test, collect a blood sample by attaching a needle to a standard blood collection tube holder. Insert the GELMATE tube into the holder and, via venipuncture, draw whole blood into the tube to the level of the blue line. The vacuum is adjusted so that the correct amount of blood will be drawn in. If this does not occur, discard the tube.

As soon as the blood enters the tube, note the time. Periodically (every minute or so) tilt the tube and observe for gel formation (the sample will turn solid). Note the time and calculate the amount of time it takes to clot.

Interpretation Of Results

1. Solid clot formation in less than five (5) minutes indicates severe inflammation.

2. Clot formation in 5-10 minutes indicates moderate inflammation.

3. Clot formation in 10-15 minutes indicates mild inflammation.

4. Clot formation in >15 minutes represents essentially normal conditions.

Typical Gammaglobulin and Fibrinogen Levels:

1. A clot time in less than five (5) minutes will usually be caused by fibrinogen over 700 mg/dl and gammaglobulin over 3,000 mg/dl.

2. A clot time of 5-10 minutes would have a fibrinogen of 500-700 mg/dl and a GG of 2,500 mg/dl.

3. A clot time of 10-15 minutes will have a fibrinogen of 300-500 mg/dl and a gammaglobulin level of 2,000-2,500 mg/dl.

Conditions which could cause these gammaglobulin and fibrinogen levels:

Cattle:

1. Traumatic reticuloperitonitis

2. Pleurisy

3. Purulent bronchopneumonia

4. Tuberculosis

5. Peritonitis

6. Purulent mastitis

7. Fibrinous pneumonia

Horses:

1. Pleurisy

2. Peritonitis

3. Corynebacterium pseudotuberculosis infections

4. Pelvic, liver and kidney abscesses

Pigs:

1. Abscesses

2. Infected joints

Other species: Feedback on the use of the test in other species is welcome and where possible back-up laboratory analysis on blood samples showing a rapid gel time will be provided. Call (800) 654-9743 for details. Because the test is not species specific, it will be helpful in any animal.

Storage

Store at 4°C.

Gelmate Caution(s)

False readings can occur in cases of severe anaemia (excessive plasma) or severe dehydration (abnormally low level of plasma).

A cow with a normal appetite will sometimes show a rapid gel time if there is an internal problem which is completely walled off. These cows are not likely to perform at optimal levels if expected to produce milk. These animals will often show normal temperatures and white cell counts.

References

Available upon request.

Distributed By

Centaur V.A.

Presentation

10 tests per kit.

Nac No.

12530040