DermaTect Kit

Manufacturer: Butler

Active Ingredient(s)

Ingredients per liter of demineralized water:

Dermatophyte test medium (base):

Peptone 110	10.0 g
Dextrose	10.0 g
Agar	20.0 g
Phenol red	0.2 g
Cycloheximide	0.5 g

The pH is 5.5 ± 0.2 at 25°C.

Pre-form dermatophyte test medium contains:

Gentamicin	0.1 g
Chlortetracycline	0.1 g

DermaTect Kit Indications

DERMATECT is a formulation for dermatophytes with a yellow to red pH indicator for the growth of dermatophytes and related fungi. Gentamicin and chlortetracycline retard the growth of most bacteria, while cycloheximide inhibits the development of most saprophytic fungi.

Test Procedure

The specimen should be inoculated immediately onto the DERMATECT vial. Cutaneous specimens should be implanted by pressing the samples into the agar surface. Incubation at temperatures of 20°-30°C is satisfactory for the growth of most dermatophytes. Optimal growth and indicator change occurs at 29°C. To ensure the presence of an aerobic environment for the best recovery of dermatophytes, do not tighten the tube caps.

After 24 hours the test media may be examined for a color change from yellow to red. Most pathogenic dermatophytes will produce full color changes in three (3) to six (6) days. Other organisms may grow on DERMATECT but can be recognized as non-dermatophytes by the absence of color change or by their colonial appearance.

Inoculation onto a conventional cultivation medium is recommended for the identification of species when DERMATECT suggests the presence of a dermatophyte.

Test characteristics:

Organisms	Results
Aspergillus flavus	Suppressed
Microsporum audouinil	Growth alkaline (red)
Trichophyton rubeum	Growth, alkaline (red)

DermaTect Kit Caution(s)

For veterinary use only.

Presentation

10 and 20 vial kits.

Nac No.

10822540