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Pronunciation: nah-kā-nē′shē
Definition: a method for vital staining of bacteria in which a slide is treated with hot methylene blue solution until it acquires a sky-blue color, after which a drop of an emulsion of the bacteria is put on the cover glass and the latter laid on the slide; the bacteria are stained differentially, some parts more intensely than others.
Examples: glitazone, GI cocktail, etc.
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